Lipid Phase Transition in Planar Bilayer Membrane and Its Effect on Carrier- and Pore-mediated Ion Transport

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1980 Jun 1

PMID 6158046

Citations 41

Authors

G Boheim

W Hanke

H Eibl

Affiliations

Soon will be listed here.

Abstract

Using mixed-chain lipids, we have recorded cooling and heating curves of planar bilayer membranes while they passed the lipid phase transition range. With unmodified planar bilayers, spontaneous current fluctuations are observed near the lipid phase transition temperature (tc approximately 29 degrees C). This effect coincides with the expected and measured decrease in membrane capacitance. Carrier (valinomycin)-modified planar bilayers exhibit near tc an abrupt change from a high-conducting state above tc to the state of bare membrane conductance below tc. In contrast to this behavior, planar bilayers modified by pore-forming antibiotics (gramicidin A, alamethicin) do not show any peculiar effect at tc. However, at 22--23 degrees C a pronounced maximum in pore-induced conductance is seen. Whereas the gramicidin A pore abruptly stops stepwise fluctuations below approximately 16 degrees C, with alamethicin a few long-lasting pore and pore state fluctuations persist down to 10 degrees C. It is suggested that the carrier may freeze out into the membrane/water interface. The effects observed with pore-forming substances, on the other hand, are interpreted in terms of lateral phase separation into pure lipid and lipid/antibiotic domains.

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References

Chapman D . Phase transitions and fluidity characteristics of lipids and cell membranes. Q Rev Biophys. 1975; 8(2):185-235. DOI: 10.1017/s0033583500001797. View

Lauger P . Carrier-mediated ion transport. Science. 1972; 178(4056):24-30. DOI: 10.1126/science.178.4056.24. View

Eibl H, Arnold D, Weltzien H, Westphal O . [On the synthesis of alpha and beta lecithins and their ether analogs]. Justus Liebigs Ann Chem. 1967; 709:226-30. DOI: 10.1002/jlac.19677090124. View

Mueller P, RUDIN D . Action potentials induced in biomolecular lipid membranes. Nature. 1968; 217(5130):713-9. DOI: 10.1038/217713a0. View

Pagano R, Cherry R, Chapman D . Phase transitions and heterogeneity in lipid bilayers. Science. 1973; 181(4099):557-9. DOI: 10.1126/science.181.4099.557. View

Veatch W, Mathies R, Eisenberg M, Stryer L . Simultaneous fluorescence and conductance studies of planar bilayer membranes containing a highly active and fluorescent analog of gramicidin A. J Mol Biol. 1975; 99(1):75-92. DOI: 10.1016/s0022-2836(75)80160-4. View

Ehrenstein G, Lecar H . Electrically gated ionic channels in lipid bilayers. Q Rev Biophys. 1977; 10(1):1-34. DOI: 10.1017/s0033583500000123. View

Marsh D, Watts A, Knowles P . Evidence for phase boundary lipid. Permeability of Tempo-choline into dimyristoylphosphatidylcholine vesicles at the phase transition. Biochemistry. 1976; 15(16):3570-8. DOI: 10.1021/bi00661a027. View

Papahadjopoulos D, Jacobson K, Nir S, Isac T . Phase transitions in phospholipid vesicles. Fluorescence polarization and permeability measurements concerning the effect of temperature and cholesterol. Biochim Biophys Acta. 1973; 311(3):330-48. DOI: 10.1016/0005-2736(73)90314-3. View

10.

Krasne S, Eisenman G, Szabo G . Freezing and melting of lipid bilayers and the mode of action of nonactin, valinomycin, and gramicidin. Science. 1971; 174(4007):412-5. DOI: 10.1126/science.174.4007.412. View

11.

Lau A, Chan S . Nuclear magnetic resonance studies of the interaction of alamethicin with lecithin bilayers. Biochemistry. 1974; 13(24):4942-8. DOI: 10.1021/bi00721a010. View

12.

Trauble H . [Phase transitions in lipids. Possible switch processes in biological membranes]. Naturwissenschaften. 1971; 58(6):277-84. DOI: 10.1007/BF00624732. View

13.

Haydon D, HLADKY S . Ion transport across thin lipid membranes: a critical discussion of mechanisms in selected systems. Q Rev Biophys. 1972; 5(2):187-282. DOI: 10.1017/s0033583500000883. View

14.

Hsu M, Chan S . Nuclear magnetic resonance studies of the interaction of valinomycin with unsonicated lecithin bilayers. Biochemistry. 1973; 12(20):3872-6. DOI: 10.1021/bi00744a012. View

15.

Bamberg E, Lauger P . Channel formation kinetics of gramicidin A in lipid bilayer membranes. J Membr Biol. 1973; 11(2):177-94. DOI: 10.1007/BF01869820. View

16.

Schindler H, Rosenbusch J . Matrix protein from Escherichia coli outer membranes forms voltage-controlled channels in lipid bilayers. Proc Natl Acad Sci U S A. 1978; 75(8):3751-5. PMC: 392864. DOI: 10.1073/pnas.75.8.3751. View

17.

Montal M, Mueller P . Formation of bimolecular membranes from lipid monolayers and a study of their electrical properties. Proc Natl Acad Sci U S A. 1972; 69(12):3561-6. PMC: 389821. DOI: 10.1073/pnas.69.12.3561. View

18.

Hartmann W, Galla H, Sackmann E . Polymyxin binding to charged lipid membranes. An example of cooperative lipid-protein interaction. Biochim Biophys Acta. 1978; 510(1):124-39. DOI: 10.1016/0005-2736(78)90135-9. View

19.

Marcelja S, Wolfe J . Properties of bilayer membranes in the phase transition or phase separation region. Biochim Biophys Acta. 1979; 557(1):24-31. DOI: 10.1016/0005-2736(79)90086-5. View

20.

Keough K, Davis P . Gel to liquid-crystalline phase transitions in water dispersions of saturated mixed-acid phosphatidylcholines. Biochemistry. 1979; 18(8):1453-9. DOI: 10.1021/bi00575a011. View