Reversible Control of Current Across Lipid Membranes by Local Heating

Overview

Journal Sci Rep

Specialty Science

Date 2016 Mar 5

PMID 26940847

Citations 8

Authors

Patrick Urban

Silke R Kirchner

Christian Muhlbauer

Theobald Lohmuller

Jochen Feldmann

Affiliations

Soon will be listed here.

Abstract

Lipid membranes are almost impermeable for charged molecules and ions that can pass the membrane barrier only with the help of specialized transport proteins. Here, we report how temperature manipulation at the nanoscale can be employed to reversibly control the electrical resistance and the amount of current that flows through a bilayer membrane with pA resolution. For this experiment, heating is achieved by irradiating gold nanoparticles that are attached to the bilayer membrane with laser light at their plasmon resonance frequency. We found that controlling the temperature on the nanoscale renders it possible to reproducibly regulate the current across a phospholipid membrane and the membrane of living cells in absence of any ion channels.

Citing Articles

Nanoparticle-based optical interfaces for retinal neuromodulation: a review.

Stoddart P, Begeng J, Tong W, Ibbotson M, Kameneva T Front Cell Neurosci. 2024; 18:1360870.

PMID: 38572073 PMC: 10987880. DOI: 10.3389/fncel.2024.1360870.

Exploring the Bottom-Up Growth of Anisotropic Gold Nanoparticles from Substrate-Bound Seeds in Microfluidic Reactors.

Vinnacombe-Willson G, Lee J, Chiang N, Scarabelli L, Yue S, Foley R ACS Appl Nano Mater. 2023; 6(8):6454-6460.

PMID: 37152920 PMC: 10152454. DOI: 10.1021/acsanm.3c00440.

Modeling the gene delivery process of the needle array-based tissue nanotransfection.

Li Z, Xuan Y, Ghatak S, Guda P, Roy S, Sen C Nano Res. 2022; 15(4):3409-3421.

PMID: 36275042 PMC: 9581438. DOI: 10.1007/s12274-021-3947-1.

Modulation of Photoinduced Transmembrane Currents in a Fullerene-Doped Freestanding Lipid Bilayer by a Lateral Bias.

Ma T, Feng X, Ohori T, Miyata R, Tadaki D, Yamaura D ACS Omega. 2019; 4(19):18299-18303.

PMID: 31720530 PMC: 6844088. DOI: 10.1021/acsomega.9b02336.

Hierarchy of Hybrid Materials-The Place of Inorganics--Organics in it, Their Composition and Applications.

Saveleva M, Eftekhari K, Abalymov A, Douglas T, Volodkin D, Parakhonskiy B Front Chem. 2019; 7:179.

PMID: 31019908 PMC: 6459030. DOI: 10.3389/fchem.2019.00179.

References

Dietrich C, Bagatolli L, Volovyk Z, Thompson N, Levi M, Jacobson K . Lipid rafts reconstituted in model membranes. Biophys J. 2001; 80(3):1417-28. PMC: 1301333. DOI: 10.1016/S0006-3495(01)76114-0. View

Wilson M, Pohorille A . Mechanism of unassisted ion transport across membrane bilayers. J Am Chem Soc. 1996; 118(28):6580-7. DOI: 10.1021/ja9540381. View

Ohvo-Rekila H, Ramstedt B, Leppimaki P, Peter Slotte J . Cholesterol interactions with phospholipids in membranes. Prog Lipid Res. 2001; 41(1):66-97. DOI: 10.1016/s0163-7827(01)00020-0. View

Fertig N, Blick R, Behrends J . Whole cell patch clamp recording performed on a planar glass chip. Biophys J. 2002; 82(6):3056-62. PMC: 1302093. DOI: 10.1016/S0006-3495(02)75646-4. View

Perozo E, Kloda A, Cortes D, Martinac B . Physical principles underlying the transduction of bilayer deformation forces during mechanosensitive channel gating. Nat Struct Biol. 2002; 9(9):696-703. DOI: 10.1038/nsb827. View

Edidin M . Lipids on the frontier: a century of cell-membrane bilayers. Nat Rev Mol Cell Biol. 2003; 4(5):414-8. DOI: 10.1038/nrm1102. View

Martinac B . Mechanosensitive ion channels: molecules of mechanotransduction. J Cell Sci. 2004; 117(Pt 12):2449-60. DOI: 10.1242/jcs.01232. View

Sands Z, Grottesi A, Sansom M . Voltage-gated ion channels. Curr Biol. 2005; 15(2):R44-7. DOI: 10.1016/j.cub.2004.12.050. View

Wells J, Kao C, Mariappan K, Albea J, Jansen E, Konrad P . Optical stimulation of neural tissue in vivo. Opt Lett. 2005; 30(5):504-6. DOI: 10.1364/ol.30.000504. View

10.

Boyden E, Zhang F, Bamberg E, Nagel G, Deisseroth K . Millisecond-timescale, genetically targeted optical control of neural activity. Nat Neurosci. 2005; 8(9):1263-8. DOI: 10.1038/nn1525. View

11.

Gouaux E, MacKinnon R . Principles of selective ion transport in channels and pumps. Science. 2005; 310(5753):1461-5. DOI: 10.1126/science.1113666. View

12.

Janshoff A, Steinem C . Transport across artificial membranes-an analytical perspective. Anal Bioanal Chem. 2006; 385(3):433-51. DOI: 10.1007/s00216-006-0305-9. View

13.

Dhaka A, Viswanath V, Patapoutian A . Trp ion channels and temperature sensation. Annu Rev Neurosci. 2006; 29:135-61. DOI: 10.1146/annurev.neuro.29.051605.112958. View

14.

Herlitze S, Landmesser L . New optical tools for controlling neuronal activity. Curr Opin Neurobiol. 2006; 17(1):87-94. DOI: 10.1016/j.conb.2006.12.002. View

15.

Olapinski M, Manus S, Fertig N, Simmel F . Probing whole cell currents in high-frequency electrical fields: identification of thermal effects. Biosens Bioelectron. 2007; 23(6):872-8. DOI: 10.1016/j.bios.2007.09.004. View

16.

Richardson H, Carlson M, Tandler P, Hernandez P, Govorov A . Experimental and theoretical studies of light-to-heat conversion and collective heating effects in metal nanoparticle solutions. Nano Lett. 2009; 9(3):1139-46. PMC: 2669497. DOI: 10.1021/nl8036905. View

17.

Urban A, Fedoruk M, Horton M, Radler J, Stefani F, Feldmann J . Controlled nanometric phase transitions of phospholipid membranes by plasmonic heating of single gold nanoparticles. Nano Lett. 2009; 9(8):2903-8. DOI: 10.1021/nl901201h. View

18.

Baffou G, Quidant R, de Abajo F . Nanoscale control of optical heating in complex plasmonic systems. ACS Nano. 2010; 4(2):709-16. DOI: 10.1021/nn901144d. View

19.

Janovjak H, Szobota S, Wyart C, Trauner D, Isacoff E . A light-gated, potassium-selective glutamate receptor for the optical inhibition of neuronal firing. Nat Neurosci. 2010; 13(8):1027-32. PMC: 2915903. DOI: 10.1038/nn.2589. View

20.

Urban A, Lutich A, Stefani F, Feldmann J . Laser printing single gold nanoparticles. Nano Lett. 2010; 10(12):4794-8. DOI: 10.1021/nl1030425. View