Phase Separation in Biological Membranes: Integration of Theory and Experiment

Overview

Journal Annu Rev Biophys

Publisher Annual Reviews

Specialty Biophysics

Date 2010 Mar 3

PMID 20192775

Citations 61

Authors

Elliot L Elson

Eliot Fried

John E Dolbow

Guy M Genin

Affiliations

Soon will be listed here.

Abstract

Lipid bilayer model membranes that contain a single lipid species can undergo transitions between ordered and disordered phases, and membranes that contain a mixture of lipid species can undergo phase separations. Studies of these transformations are of interest for what they can tell us about the interaction energies of lipid molecules of different species and conformations. Nanoscopic phases (<200 nm) can provide a model for membrane rafts, specialized membrane domains enriched in cholesterol and sphingomyelin, which are believed to have essential biological functions in cell membranes. Crucial questions are whether lipid nanodomains can exist in stable equilibrium in membranes and what is the distribution of their sizes and lifetimes in membranes of different composition. Theoretical methods have supplied much information on these questions, but better experimental methods are needed to detect and characterize nanodomains under normal membrane conditions. This review summarizes linkages between theoretical and experimental studies of phase separation in lipid bilayer model membranes.

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