Complete Nucleotide Sequence of a 23S Ribosomal RNA Gene from Escherichia Coli

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1980 Jan 1

PMID 6153795

Citations 162

Authors

J Brosius

T J Dull

H F Noller

Affiliations

Soon will be listed here.

Abstract

The complete nucleotide sequence of the 23S RNA gene from the rrnB operon of Escherichia coli has been determined. The sequences of both strands of the entire gene were determined, most of the sequence was independently confirmed by use of alternate restriction fragments, and all restriction cuts overlapped. The DNA region corresponding to mature 23S rRNA contains 2904 nucleotides. Kethoxal-reactive sites protected by 30S subunits are found between positions 2300 and 28000, placing the subunit interface in this region of the molecule. The functional importance of this region is further supported by studies by other investigators, including homology with chloroplast and mitochondrial rRNA.

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References

Stanley Jr W, Bock R . Isolation and physical properties of the ribosomal ribonucleic acid of Escherichia coli. Biochemistry. 1965; 4(7):1302-11. DOI: 10.1021/bi00883a014. View

Sharp P, Sugden B, Sambrook J . Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis. Biochemistry. 1973; 12(16):3055-63. DOI: 10.1021/bi00740a018. View

Brosius J, Palmer M, Kennedy P, Noller H . Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci U S A. 1978; 75(10):4801-5. PMC: 336208. DOI: 10.1073/pnas.75.10.4801. View

Bernardi A, Bernardi F . Construction in vitro of hybrid plasmids carrying all the EcoRI fragments from lambdarifd18 DNA. Eur J Biochem. 1979; 95(2):391-8. DOI: 10.1111/j.1432-1033.1979.tb12977.x. View

Fellner P, Ebel J, Blasi O . The 3'-terminal nucleotide sequence of the 23 S ribosomal RNA from Escherichia coli. FEBS Lett. 1970; 6(2):102-104. DOI: 10.1016/0014-5793(70)80012-6. View

Fernandez-Munoz R, MONRO R, Vazquez D . Substrate- and antibiotic-binding sites at the peptidyl-transferase centre of Escherichia coli ribosomes. Studies on the chloramphenicol. lincomycin and erythromycin sites. Eur J Biochem. 1971; 23(1):185-93. DOI: 10.1111/j.1432-1033.1971.tb01607.x. View

Pestka S . Studies on the formation of transfer ribonucleic acid-ribosome complexes. XI. Antibiotic effects on phenylalanyl-oligonucleotide binding to ribosomes. Proc Natl Acad Sci U S A. 1969; 64(2):709-14. PMC: 223402. DOI: 10.1073/pnas.64.2.709. View

Miyajima A, Shibuya M, Kaziro Y . Construction and characterization of the two hybrid Co1E1 plasmids carrying Escherichia coli tufB gene. FEBS Lett. 1979; 102(2):207-10. DOI: 10.1016/0014-5793(79)80001-0. View

Dahlberg J, Kintner C, Lund E . Specific binding of tRNAMet to 23S rRNA of Escherichia coli. Proc Natl Acad Sci U S A. 1978; 75(3):1071-5. PMC: 411410. DOI: 10.1073/pnas.75.3.1071. View

10.

Bolivar F, Rodriguez R, Betlach M, Boyer H . Construction and characterization of new cloning vehicles. I. Ampicillin-resistant derivatives of the plasmid pMB9. Gene. 1977; 2(2):75-93. DOI: 10.1016/0378-1119(77)90074-9. View

11.

Allet B, Rochaix J . Structure analysis at the ends of the intervening DNA sequences in the chloroplast 23S ribosomal genes of C. reinhardii. Cell. 1979; 18(1):55-60. DOI: 10.1016/0092-8674(79)90353-2. View

12.

Herr W, Noller H . Protection of specific sites in 23 S and 5 S RNA from chemical modification by association of 30 S and 50 S ribosomes. J Mol Biol. 1979; 130(4):421-32. DOI: 10.1016/0022-2836(79)90432-7. View

13.

Palmer M, Raker M, Kennedy P, Young J, Barnes W, Rodriguez R . Isolation and restriction mapping of plasmids containing ribosomal DNA sequences from the rrn B cistron of E. coli. Mol Gen Genet. 1979; 172(2):171-8. DOI: 10.1007/BF00268279. View

14.

Herr W, Noller H . A fragment of 23S RNA containing a nucleotide sequence complementary to a region of 5S RNA. FEBS Lett. 1975; 53(2):248-52. DOI: 10.1016/0014-5793(75)80030-5. View

15.

SANGER F, Coulson A . The use of thin acrylamide gels for DNA sequencing. FEBS Lett. 1978; 87(1):107-10. DOI: 10.1016/0014-5793(78)80145-8. View

16.

Carbon P, Ehresmann C, Ehresmann B, Ebel J . The sequence of Escherichia coli ribosomal 16 S RNA determined by new rapid gel methods. FEBS Lett. 1978; 94(1):152-6. DOI: 10.1016/0014-5793(78)80926-0. View

17.

Szalay A, Grohmann K, SINSHEIMER R . Separation of the complementary strands of DNA fragments on polyacrylamide gels. Nucleic Acids Res. 1977; 4(5):1569-78. PMC: 343774. DOI: 10.1093/nar/4.5.1569. View

18.

Midgley J . The estimation of polynucleotide chain length by a chemical method. Biochim Biophys Acta. 1965; 108(3):340-7. DOI: 10.1016/0005-2787(65)90026-2. View

19.

Sekiya T, Nishimura S . Sequence of the gene for isoleucine tRNA1 and the surrounding region in a ribosomal RNA operon of Escherichia coli. Nucleic Acids Res. 1979; 6(2):575-92. PMC: 327714. DOI: 10.1093/nar/6.2.575. View

20.

Allet B, SPAHR P . Binding sites of ribosomal proteins on two specific fragments derived from Escherichia coli 50 S ribosomes. Eur J Biochem. 1971; 19(2):250-5. DOI: 10.1111/j.1432-1033.1971.tb01311.x. View