Transcription of Ribosomal DNA in Chloroplasts of Spirodela Oligorhizaa

Overview

Journal Curr Genet

Specialty Genetics

Date 2013 Nov 1

PMID 24173515

Citations 10

Authors

R J Keus

A F Dekker

K C Kreuk

G S Groot

Affiliations

Soon will be listed here.

Abstract

The genes for the two large ribosomal RNAs (16S and 23S) and for the 4.5S rRNA in Spirodela oligorhiza chloroplast DNA are transcribed as one large, 7,000 nucleotides long precursor rRNA.Using S1-nuclease mapping, we have determined that the transcript ends 135 nucleotides 3' distal of the 4.5S rRNA gene. 5S rRNA therefore, is most likely transcribed separately.Northern blotting of chloroplast RNA with distinct probes derived from the rDNA region reveals RNAs, which can be described as intermediates in the processing of the large precursor. With these findings a pathway for the maturation of this precursor is proposed.

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