Transmembrane Electrical Potential in Rickettsia Prowazekii and Its Relationship to Lysine Transport

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1983 Feb 1

PMID 6130061

Citations 11

Authors

R J Zahorchak

H H Winkler

Affiliations

Soon will be listed here.

Abstract

The transmembrane electrical potential (delta psi) generated by Rickettsia prowazekii metabolizing glutamic acid or ATP was determined by flow dialysis with the lipophilic cation tetraphenylphosphonium and with lysine. At pH 7.0, the rickettsiae generated a delta psi as measured by tetraphenylphosphonium distribution of 90 mV. Under similar conditions, cells of R.prowazekii concentrated lysine to a gradient indicating a delta psi of 90 mV. Energy-starved cells of R. prowazekii were able to utilize exogenously supplied ATP as well as glutamic acid to generate a delta psi of 110 mV at pH 8.0. Lysine transport was markedly affected by environmental pH, the optimum pH ranging from 8.0 to 8.5. delta psi as measured with tetraphenyl-phosphonium was similarly affected in this system, with values ranging from 70 mV at pH 6.0 to 100 mV at pH 8.0. Respiration rates were also affected by the external pH, with a maximum rate of 28 nmol of O2 consumed per min per mg of rickettsial protein occurring at pH 8.0. The pH effects were readily reversible and with a rapid onset.

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References

Markwell M, Haas S, Bieber L, Tolbert N . A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem. 1978; 87(1):206-10. DOI: 10.1016/0003-2697(78)90586-9. View

Myers W, Provost P, WISSEMAN Jr C . Permeability properties of Rickettsia mooseri. J Bacteriol. 1967; 93(3):950-60. PMC: 276540. DOI: 10.1128/jb.93.3.950-960.1967. View

Harold F . Conservation and transformation of energy by bacterial membranes. Bacteriol Rev. 1972; 36(2):172-230. PMC: 408323. DOI: 10.1128/br.36.2.172-230.1972. View

Maloney P . Coupling between H+ entry and ATP formation in Escherichia coli. Biochem Biophys Res Commun. 1978; 83(4):1496-501. DOI: 10.1016/0006-291x(78)91390-6. View

Winkler H . Rickettsial permeability. An ADP-ATP transport system. J Biol Chem. 1976; 251(2):389-96. View

Weiss E, Coolbaugh J, Williams J . Separation of viable Rickettsia typhi from yolk sac and L cell host components by renografin density gradient centrifugation. Appl Microbiol. 1975; 30(3):456-63. PMC: 187203. DOI: 10.1128/am.30.3.456-463.1975. View

Winkler H . Rickettsial cell water and membrane permeability determined by a micro space technique. Appl Environ Microbiol. 1976; 31(1):146-9. PMC: 169733. DOI: 10.1128/aem.31.1.146-149.1976. View

Padan E, Zilberstein D, Rottenberg H . The proton electrochemical gradient in Escherichia coli cells. Eur J Biochem. 1976; 63(2):533-41. DOI: 10.1111/j.1432-1033.1976.tb10257.x. View

Mitchell P . Chemiosmotic coupling in oxidative and photosynthetic phosphorylation. Biol Rev Camb Philos Soc. 1966; 41(3):445-502. DOI: 10.1111/j.1469-185x.1966.tb01501.x. View

10.

Ramos S, Schuldiner S, Kaback H . The electrochemical gradient of protons and its relationship to active transport in Escherichia coli membrane vesicles. Proc Natl Acad Sci U S A. 1976; 73(6):1892-6. PMC: 430413. DOI: 10.1073/pnas.73.6.1892. View

11.

Guffanti A, Susman P, Blanco R, Krulwich T . The protonmotive force and alpha-aminoisobutyric acid transport in an obligately alkalophilic bacterium. J Biol Chem. 1978; 253(3):708-15. View

12.

Walker T, Winkler H . Rickettsial hemolysis: rapid method for enumeration of metabolically active typhus rickettsiae. J Clin Microbiol. 1979; 9(5):645-7. PMC: 275367. DOI: 10.1128/jcm.9.5.645-647.1979. View

13.

Niven D, Jeacocke R, Hamilton W . The membrane potential as the driving force for the accumulation of lysine by Staphylococcus aureus. FEBS Lett. 1973; 29(3):248-252. DOI: 10.1016/0014-5793(73)80030-4. View

14.

Williams J, Weiss E . Energy metabolism of Rickettsia typhi: pools of adenine nucleotides and energy charge in the presence and absence of glutamate. J Bacteriol. 1978; 134(3):884-92. PMC: 222335. DOI: 10.1128/jb.134.3.884-892.1978. View

15.

Wilson D, Alderette J, Maloney P, Wilson T . Protonmotive force as the source of energy for adenosine 5'-triphosphate synthesis in Escherichia coli. J Bacteriol. 1976; 126(1):327-37. PMC: 233290. DOI: 10.1128/jb.126.1.327-337.1976. View

16.

Wike D, TALLENT G, Peacock M, ORMSBEE R . Studies of the rickettsial plaque assay technique. Infect Immun. 1972; 5(5):715-22. PMC: 422430. DOI: 10.1128/iai.5.5.715-722.1972. View

17.

BOVARNICK M . Phosphorylation accompanying the oxidation of glutamate by the Madrid E strain of typhus rickettsiae. J Biol Chem. 1956; 220(1):353-61. View

18.

Haddock B, Jones C . Bacterial respiration. Bacteriol Rev. 1977; 41(1):47-99. PMC: 413996. DOI: 10.1128/br.41.1.47-99.1977. View

19.

Smith D, Winkler H . Separation of inner and outer membranes of Rickettsia prowazeki and characterization of their polypeptide compositions. J Bacteriol. 1979; 137(2):963-71. PMC: 218381. DOI: 10.1128/jb.137.2.963-971.1979. View

20.

Winkler H . Inhibitory and restorative effects of adenine nucleotides on rickettsial adsorption and hemolysis. Infect Immun. 1974; 9(1):119-26. PMC: 414775. DOI: 10.1128/iai.9.1.119-126.1974. View