Transcriptional Regulation in the Obligate Intracytoplasmic Bacterium Rickettsia Prowazekii

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1996 Sep 1

PMID 8808950

Citations 9

Authors

J Cai

H H Winkler

Affiliations

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Abstract

Transcriptional regulation was demonstrated in Rickettsia prowazekii, an obligate intracytoplasmic bacterium. The level of citrate synthase (gltA) mRNA II, from promoter P2, was greater in the total RNA isolated from heavily infected L929 cells than in moderately infected L929 cells; conversely, the level of ATP/ADP translocase (tlc) mRNA was greater in moderately infected cells. The level of gltA mRNA I, from promoter P1, did not change under these conditions. The chemical half-lives of gltA mRNA II and tlc mRNA under these conditions were very similar.

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References

Winkler H . Rickettsial permeability. An ADP-ATP transport system. J Biol Chem. 1976; 251(2):389-96. View

Weitzman P, Danson M . Citrate synthase. Curr Top Cell Regul. 1976; 10:161-204. DOI: 10.1016/b978-0-12-152810-2.50011-5. View

Phibbs Jr P, Winkler H . Regulatory properties of citrate synthase from Rickettsia prowazekii. J Bacteriol. 1982; 149(2):718-25. PMC: 216564. DOI: 10.1128/jb.149.2.718-725.1982. View

Weitzman P . Unity and diversity in some bacterial citric acid-cycle enzymes. Adv Microb Physiol. 1981; 22:185-244. DOI: 10.1016/s0065-2911(08)60328-8. View

Zahorchak R, Winkler H . Transmembrane electrical potential in Rickettsia prowazekii and its relationship to lysine transport. J Bacteriol. 1983; 153(2):665-71. PMC: 221683. DOI: 10.1128/jb.153.2.665-671.1983. View

Wood D, Atkinson W, Sikorski R, Winkler H . Expression of the Rickettsia prowazekii citrate synthase gene in Escherichia coli. J Bacteriol. 1983; 155(1):412-6. PMC: 217695. DOI: 10.1128/jb.155.1.412-416.1983. View

Winkler H, DAUGHERTY R . Regulatory role of phosphate and other anions in transport of ADP and ATP by Rickettsia prowazekii. J Bacteriol. 1984; 160(1):76-9. PMC: 214683. DOI: 10.1128/jb.160.1.76-79.1984. View

Krause D, Winkler H, Wood D . Cloning and expression of the Rickettsia prowazekii ADP/ATP translocator in Escherichia coli. Proc Natl Acad Sci U S A. 1985; 82(9):3015-9. PMC: 397697. DOI: 10.1073/pnas.82.9.3015. View

Wiegand G, Remington S . Citrate synthase: structure, control, and mechanism. Annu Rev Biophys Biophys Chem. 1986; 15:97-117. DOI: 10.1146/annurev.bb.15.060186.000525. View

10.

Wood D, Williamson L, Winkler H, Krause D . Nucleotide sequence of the Rickettsia prowazekii citrate synthase gene. J Bacteriol. 1987; 169(8):3564-72. PMC: 212433. DOI: 10.1128/jb.169.8.3564-3572.1987. View

11.

Wilde R, Guest J . Transcript analysis of the citrate synthase and succinate dehydrogenase genes of Escherichia coli K12. J Gen Microbiol. 1986; 132(12):3239-51. DOI: 10.1099/00221287-132-12-3239. View

12.

Williamson L, Plano G, Winkler H, Krause D, Wood D . Nucleotide sequence of the Rickettsia prowazekii ATP/ADP translocase-encoding gene. Gene. 1989; 80(2):269-78. DOI: 10.1016/0378-1119(89)90291-6. View

13.

Fouet A, Sonenshein A . A target for carbon source-dependent negative regulation of the citB promoter of Bacillus subtilis. J Bacteriol. 1990; 172(2):835-44. PMC: 208513. DOI: 10.1128/jb.172.2.835-844.1990. View

14.

Plano G, Wood D, Winkler H . Rickettsia prowazekii and ATP/ADP translocase. Analysis of gene fusions encoding beta-galactosidase-ATP/ADP translocase fusion proteins. Ann N Y Acad Sci. 1990; 590:397-407. DOI: 10.1111/j.1749-6632.1990.tb42247.x. View

15.

Winkler H . Rickettsia species (as organisms). Annu Rev Microbiol. 1990; 44:131-53. DOI: 10.1146/annurev.mi.44.100190.001023. View

16.

Cai J, Speed R, Winkler H . Reduction of ribonucleotides by the obligate intracytoplasmic bacterium Rickettsia prowazekii. J Bacteriol. 1991; 173(4):1471-7. PMC: 207284. DOI: 10.1128/jb.173.4.1471-1477.1991. View

17.

Cai J, Winkler H . Identification of tlc and gltA mRNAs and determination of in situ RNA half-life in Rickettsia prowazekii. J Bacteriol. 1993; 175(17):5725-7. PMC: 206636. DOI: 10.1128/jb.175.17.5725-5727.1993. View

18.

Perez-Martin J, Rojo F, de Lorenzo V . Promoters responsive to DNA bending: a common theme in prokaryotic gene expression. Microbiol Rev. 1994; 58(2):268-90. PMC: 372964. DOI: 10.1128/mr.58.2.268-290.1994. View

19.

Park S, McCabe J, Turna J, Gunsalus R . Regulation of the citrate synthase (gltA) gene of Escherichia coli in response to anaerobiosis and carbon supply: role of the arcA gene product. J Bacteriol. 1994; 176(16):5086-92. PMC: 196348. DOI: 10.1128/jb.176.16.5086-5092.1994. View

20.

Jin S, Sonenshein A . Transcriptional regulation of Bacillus subtilis citrate synthase genes. J Bacteriol. 1994; 176(15):4680-90. PMC: 196290. DOI: 10.1128/jb.176.15.4680-4690.1994. View