Rickettsial Hemolysis: Rapid Method for Enumeration of Metabolically Active Typhus Rickettsiae

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1979 May 1

PMID 113426

Citations 33

Authors

T S Walker

H H Winkler

Affiliations

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Abstract

A new assay is described for enumerating biologically active typhus rickettsiae (Madrid E strain), based on adsorption of rickettsiae to erythrocytes in the presence of NaF (which allows adsorption but not lysis) and lysis in the presence of anti-Rickettsia prowazeki immune serum (which allows only a single round of lysis). The number of lysed erythrocytes is then used to estimate the number of active rickettsiae.

Citing Articles

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Dual mechanisms of metabolite acquisition by the obligate intracytosolic pathogen Rickettsia prowazekii reveal novel aspects of triose phosphate transport.

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Rickettsia prowazekii uses an sn-glycerol-3-phosphate dehydrogenase and a novel dihydroxyacetone phosphate transport system to supply triose phosphate for phospholipid biosynthesis.

Frohlich K, Roberts R, Housley N, Audia J J Bacteriol. 2010; 192(17):4281-8.

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Nitric oxide-mediated inhibition of the ability of Rickettsia prowazekii to infect mouse fibroblasts and mouse macrophagelike cells.

Turco J, Liu H, Gottlieb S, Winkler H Infect Immun. 1998; 66(2):558-66.

PMID: 9453609 PMC: 107941. DOI: 10.1128/IAI.66.2.558-566.1998.

Rickettsial stimulation of endothelial platelet-activating factor synthesis.

Walker T, Mellott G Infect Immun. 1993; 61(5):2024-9.

PMID: 8478089 PMC: 280798. DOI: 10.1128/iai.61.5.2024-2029.1993.

References

WISSEMAN Jr C, Jackson E, Hahn F, Ley A, SMADEL J . Metabolic studies of rickettsiae. I. The effects of antimicrobial substances and enzyme inhibitors on the oxidation of glutamate by purified rickettsiae. J Immunol. 1951; 67(2):123-36. View

WISSEMAN Jr C, Waddell A, Silverman D . In vitro studies on Rickettsia-host cell interactions: lag phase in intracellular growth cycle as a function of stage of growth of infecting Rickettsia prowazeki, with preliminary observations on inhibition of rickettsial uptake by host cell.... Infect Immun. 1976; 13(6):1749-60. PMC: 420828. DOI: 10.1128/iai.13.6.1749-1760.1976. View

Winkler H . Rickettsial hemolysis: adsorption, desorption, readsorption, and hemagglutination. Infect Immun. 1977; 17(3):607-12. PMC: 421170. DOI: 10.1128/iai.17.3.607-612.1977. View

Ramm L, Winkler H . Rickettsial hemolysis: effect of metabolic inhibitors upon hemolysis and adsorption. Infect Immun. 1973; 7(4):550-5. PMC: 422720. DOI: 10.1128/iai.7.4.550-555.1973. View

Wike D, TALLENT G, Peacock M, ORMSBEE R . Studies of the rickettsial plaque assay technique. Infect Immun. 1972; 5(5):715-22. PMC: 422430. DOI: 10.1128/iai.5.5.715-722.1972. View