Identification of Thymocyte Progenitors in Hemopoietic Tissues of the Rat. II. Enrichment of Functional Prothymocytes on the Fluorescence-activated Cell Sorter

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1982 Nov 1

PMID 6127371

Citations 5

Authors

D L Greiner

I Goldschneider

R W Barton

Affiliations

Soon will be listed here.

Abstract

A quantitative thymocyte regeneration assay was used to monitor the isolation of functional prothymocytes from rat bone marrow on the FACS. Two prothymocyte subpopulations were tentatively identified on the basis of their relative resistance to dexamethasone. Both populations were comprised of undifferentiated, medium-size cells that displayed large amounts of Thy-1 antigen. Simultaneous sorting of bone marrow cells according to relative low angle light scatter (size) and relative fluorescence intensity for Thy-1 resulted in enrichments of 112-fold and 260-fold, respectively, in prothymocyte activity in untreated and dexamethasone-treated bone marrow. These prothymocyte-enriched cell fractions contained or approximately 75% of total functional prothymocyte activity in bone marrow, and represented 1.1 and 0.35% of total untreated and dexamethasone-treated bone marrow cells. Using these enriched cell fractions, significant thymocyte regeneration is possible with as few as 2 X 10(4) and 1 X 10(4) bone marrow cells, respectively. The possible relationship of these functional prothymocyte subpopulations with CFU-S and with TdT-positive cells is discussed.

Citing Articles

Defective lymphopoiesis in bone marrow of motheaten (me/me) and viable motheaten (mev/mev) mutant mice. I. Analysis of development of prothymocytes, early B lineage cells, and terminal deoxynucleotidyl transferase-positive cells.

Greiner D, Goldschneider I, Komschlies K, Medlock E, Bollum F, Schultz L J Exp Med. 1986; 164(4):1129-44.

PMID: 3531380 PMC: 2188409. DOI: 10.1084/jem.164.4.1129.

Studies of thymocytopoiesis in rats and mice. I. Kinetics of appearance of thymocytes using a direct intrathymic adoptive transfer assay for thymocyte precursors.

Goldschneider I, Komschlies K, Greiner D J Exp Med. 1986; 163(1):1-17.

PMID: 3510267 PMC: 2188012. DOI: 10.1084/jem.163.1.1.

Frequency of 6-thioguanine-resistant T cells is inversely related to the declining T-cell activities in aging mice.

Inamizu T, Kinohara N, Chang M, Makinodan T Proc Natl Acad Sci U S A. 1986; 83(8):2488-91.

PMID: 3486421 PMC: 323323. DOI: 10.1073/pnas.83.8.2488.

Thymotaxin: a thymic epithelial peptide chemotactic for T-cell precursors.

Imhof B, Deugnier M, Girault J, Champion S, Damais C, Itoh T Proc Natl Acad Sci U S A. 1988; 85(20):7699-703.

PMID: 3051002 PMC: 282260. DOI: 10.1073/pnas.85.20.7699.

Phenotypic and genotypic characteristics of the human prothymocyte.

van Dongen J, Comans-Bitter W Immunol Res. 1987; 6(4):250-62.

PMID: 2963079 DOI: 10.1007/BF02935519.

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