Transcriptional Control of Polarity in Escherichia Coli by CAMP

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1984 Jan 1

PMID 6092868

Citations 10

Authors

A Danchin

A Ullmann

Affiliations

Soon will be listed here.

Abstract

In Escherichia coli, 3'5'-adenosine cyclic monophosphate (cAMP) and its receptor protein (CAP) are known to be involved in the control of transcription initiation of catabolic operons. In previous papers we have shown that the cAMP-CAP complex is also involved as a modulator of polarity in polycistronic transcription units. Furthermore we showed that there exists a functional relationship between this complex and the transcription termination protein, Rho. In this work, we measured mRNA synthesis corresponding to the promoter proximal and distal parts of the lac and gal operons by DNA-RNA hybridization. We show that in these operons the main polarity effect is essentially transcriptional and the cAMP-CAP complex decreases polarity by interfering with premature transcription termination.

Citing Articles

sRNA-mediated regulation of gal mRNA in E. coli: Involvement of transcript cleavage by RNase E together with Rho-dependent transcription termination.

Jeon H, Lee Y, N M, Wang X, Chattoraj D, Lim H PLoS Genet. 2021; 17(10):e1009878.

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In vivo transcription dynamics of the galactose operon: a study on the promoter transition from P1 to P2 at onset of stationary phase.

Ji S, Wang X, Yun S, Jeon H, Lee H, Kim H PLoS One. 2011; 6(3):e17646.

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Timing of gene transcription in the galactose utilization system of Escherichia coli.

Horvath P, Hunziker A, Erdossy J, Krishna S, Semsey S J Biol Chem. 2010; 285(49):38062-8.

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Spot 42 RNA mediates discoordinate expression of the E. coli galactose operon.

Moller T, Franch T, Udesen C, Gerdes K, Valentin-Hansen P Genes Dev. 2002; 16(13):1696-706.

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Transcription analysis of the sucAB, aceEF and lpd genes of Escherichia coli.

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