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Cyclic AMP As a Modulator of Polarity in Polycistronic Transcriptional Units

Overview
Journal Proc Natl Acad Sci U S A
Specialty Science
Date 1979 Jul 1
PMID 226959
Citations 43
Authors
A Ullmann
E Joseph
A Danchin
Affiliations
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Abstract

The degree of natural polarity in the lactose and galactose operons of Escherichia coli is affected by adenosine 3',5'-cyclic monophosphate (cAMP). This effect, mediated by the cAMP receptor protein, is exerted at sites distinct from the promoter. Experiments performed with a mutant bearing a thermosensitive rho factor activity indicate that cAMP relieves polarity by interfering with transcription termination. Conflicting results in the literature concerning the role of cAMP receptor protein and cAMP in galactose operon expression can be reconciled by the findings that cAMP stimulates the expression of operator distal genes without significantly affecting the proximal genes. Therefore, it appears necessary to reevaluate the classification of the galactose operon as exhibiting cAMP-mediated catabolite repression at the level of transcription initiation.

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References
1.
Buttin G . [REGULATORY MECHANISMS IN THE BIOSYNTHESIS OF THE ENZYMES OF GALACTOSE METABOLISM IN ESCHERICHIA COLI K 12. I. THE INDUCED BIOSYNTHESIS OF GALACTOKINASE AND THE SIMULTANEOUS INDUCTION OF THE ENZYMATIC SEQUENCE]. J Mol Biol. 1963; 7:164-82. DOI: 10.1016/s0022-2836(63)80044-3. View
2.
Zurawski G, Elseviers D, Stauffer G, Yanofsky C . Translational control of transcription termination at the attenuator of the Escherichia coli tryptophan operon. Proc Natl Acad Sci U S A. 1978; 75(12):5988-92. PMC: 393102. DOI: 10.1073/pnas.75.12.5988. View
3.
Wanner B, Kodaira R, Neidhardt F . Regulation of lac operon expression: reappraisal of the theory of catabolite repression. J Bacteriol. 1978; 136(3):947-54. PMC: 218529. DOI: 10.1128/jb.136.3.947-954.1978. View
4.
Musso R, Di Lauro R, Adhya S, de Crombrugghe B . Dual control for transcription of the galactose operon by cyclic AMP and its receptor protein at two interspersed promoters. Cell. 1977; 12(3):847-54. DOI: 10.1016/0092-8674(77)90283-5. View
5.
Petersen H, Joseph E, Ullmann A, Danchin A . Formylation of initiator tRNA methionine in procaryotic protein synthesis: in vivo polarity in lactose operon expression. J Bacteriol. 1978; 135(2):453-9. PMC: 222403. DOI: 10.1128/jb.135.2.453-459.1978. View