Article: A new approach in recognition of heterochromatic regions of human chromosomes by means of restriction endonucleases

The heteromorphisms of C-band regions of human chromosomes are evaluated by means of restriction endonucleases AluI, DdeI, MboI, and RsaI. Every chromosome exhibits heteromorphic markers of the C-band regions except chromosome 8. Each enzyme was found to be highly characteristic in its staining profile, a result that clearly suggests the diversity of heterochromatin. The inherent C-band-region heterochromatin variability that is revealed by these enzymes provides a valuable tool in identifying markers as compared with other previously described techniques.

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A New Approach in Recognition of Heterochromatic Regions of Human Chromosomes by Means of Restriction Endonucleases