Cytoplasmic Structures Associated with an Arbovirus Infection: Loci of Viral Ribonucleic Acid Synthesis

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1968 Nov 1

PMID 5750316

Citations 111

Authors

P M Grimley

I K Berezesky

R M Friedman

Affiliations

Soon will be listed here.

Abstract

Unique cytoplasmic structures, herein designated as type I cytopathic vacuoles (CPV-I), are found in chick embryo cells early in the logarithmic phase of Semliki Forest virus replication. High resolution autoradiography demonstrated that the CPV-I are loci of (3)H-uridine incorporation. This evidence correlates well with previous biochemical data and electron microscopy of the subcellular fractions active in Semliki Forest virus ribonucleic acid synthesis. Origin of the CPV-I within host cell cytoplasm is confirmed by the distribution of electron-dense tracer particles and sequential ultrastructural observations.

Citing Articles

A detailed kinetic model of Eastern equine encephalitis virus replication in a susceptible host cell.

Larkin C, Dunn M, Shoemaker J, Klimstra W, Faeder J bioRxiv. 2025; .

PMID: 39764060 PMC: 11703215. DOI: 10.1101/2024.12.13.628424.

Temperature Interference on ZIKV and CHIKV Cycles in Mosquitoes and Mammalian Cells.

Salles T, Martins-Duarte E, Meneses M, Moreira M, Ferreira D, Azevedo R Pathogens. 2024; 13(9).

PMID: 39339005 PMC: 11435172. DOI: 10.3390/pathogens13090814.

fate of Chikungunya virus replication organelles.

Girard J, Le Bihan O, Lai-Kee-Him J, Girleanu M, Bernard E, Castellarin C J Virol. 2024; 98(7):e0036824.

PMID: 38940586 PMC: 11265437. DOI: 10.1128/jvi.00368-24.

Characterization of Mayaro virus (strain BeAn343102) biology in vertebrate and invertebrate cellular backgrounds.

Pujhari S, Brustolin M, Heu C, Smithwick R, Larrosa M, Hafenstein S J Gen Virol. 2022; 103(10).

PMID: 36215156 PMC: 10019088. DOI: 10.1099/jgv.0.001794.

Contribution of the Golgi apparatus in morphogenesis of a virus-induced cytopathic vacuolar system.

Sengupta R, Mihelc E, Angel S, Lanman J, Kuhn R, Stahelin R Life Sci Alliance. 2022; 5(10).

PMID: 36137747 PMC: 9500387. DOI: 10.26508/lsa.202000887.

References

Friedman R, Sonnabend J . Inhibition of interferon action by puromycin. J Immunol. 1965; 95(4):696-703. View

SIMARD R, Bernhard W . [The phenomenon of nucleolar segregation: specificity of action of certain antimetabolites]. Int J Cancer. 1966; 1(5):463-79. DOI: 10.1002/ijc.2910010506. View

Eisenstein R, Battifora H, Axelrod E . Vitamin D-actinomycin D interactions in bone. An electron microscopic study of hypertrophy and hyperinvolution of osteoblasts. Lab Invest. 1966; 15(10):1589-600. View

Acheson N, Tamm I . Replication of Semliki Forest virus: an electron microscopic study. Virology. 1967; 32(1):128-43. DOI: 10.1016/0042-6822(67)90261-9. View

Friedman R, Levy H, Carter W . Replication of semliki forest virus: three forms of viral RNA produced during infection. Proc Natl Acad Sci U S A. 1966; 56(2):440-6. PMC: 224391. DOI: 10.1073/pnas.56.2.440. View

GOSSELIN R . Kinetics of pinocytosis. Fed Proc. 1967; 26(4):987-93. View

Chain M, Doane F, McLean D . Morphological development of Chikungunya virus. Can J Microbiol. 1966; 12(5):895-900. DOI: 10.1139/m66-122. View

McGEE-RUSSELL S, Gosztonyi G . Assembly of Semliki forest virus in brain. Nature. 1967; 214(5094):1204-6. DOI: 10.1038/2141204a0. View

CHAMBERS R, Bowling M, Grimley P . Glutaraldehyde fixation in routine histopathology. Arch Pathol. 1968; 85(1):18-30. View

10.

Comings D, KAKEFUDA T . Initiation of deoxyribonucleic acid replication at the nuclear membrane in human cells. J Mol Biol. 1968; 33(1):225-9. DOI: 10.1016/0022-2836(68)90290-8. View

11.

Friedman R . Replicative intermediate of an arbovirus. J Virol. 1968; 2(6):547-52. PMC: 375647. DOI: 10.1128/JVI.2.6.547-552.1968. View

12.

Friedman R . Protein synthesis directed by an arbovirus. J Virol. 1968; 2(1):26-32. PMC: 375574. DOI: 10.1128/JVI.2.1.26-32.1968. View

13.

Erlandson R, BABCOCK V, SOUTHAM C, Bailey R, Shipkey F . Semliki Forest virus in HEp-2 cell cultures. J Virol. 1967; 1(5):996-1009. PMC: 375379. DOI: 10.1128/JVI.1.5.996-1009.1967. View

14.

Martin E, Sonnabend J . Ribonucleic acid polymerase catalyzing synthesis of double-stranded arbovirus ribonucleic acid. J Virol. 1967; 1(1):97-109. PMC: 375509. DOI: 10.1128/JVI.1.1.97-109.1967. View

15.

Friedman R, Berezesky I . Cytoplasmic fractions associated with Semliki Forest virus ribonucleic acid replication. J Virol. 1967; 1(2):374-83. PMC: 375236. DOI: 10.1128/JVI.1.2.374-383.1967. View

16.

PELC S . The stripping-film technique of autoradiography. Int J Appl Radiat Isot. 1956; 1(3):172-7. DOI: 10.1016/0020-708x(56)90003-5. View

17.

Cheng P . Purification, size, and morphology of a mosquitoborne animal virus, Semliki Forest virus. Virology. 1961; 14:124-31. DOI: 10.1016/0042-6822(61)90139-8. View

18.

Morgan C, Howe C, Rose H . Structure and development of viruses as observed in the electron microscope. V. Western equine encephalomyelitis virus. J Exp Med. 1961; 113:219-34. PMC: 2137342. DOI: 10.1084/jem.113.1.219. View

19.

LWOFF A, Horne R, Tournier P . A system of viruses. Cold Spring Harb Symp Quant Biol. 1962; 27:51-5. DOI: 10.1101/sqb.1962.027.001.008. View

20.

Caro L, VAN Tubergen R, Kolb J . High-resolution autoradiography. I. Methods. J Cell Biol. 1962; 15:173-88. PMC: 2106152. DOI: 10.1083/jcb.15.2.173. View