The Relation Between Calcium and Contraction Kinetics in Skinned Muscle Fibres

Overview

Journal J Physiol

Specialty Physiology

Date 1970 Nov 1

PMID 5500992

Citations 75

Authors

R J PODOLSKY

L E Teichholz

Affiliations

Soon will be listed here.

Abstract

1. Segments of skinned frog muscle fibres were immersed in solutions in which the calcium ion concentration, and therefore the steady isometric force, was controlled with an EGTA buffer system. The contraction kinetics were measured when the load was quickly reduced to values less than the isometric force.2. The velocity of shortening following release from steady force was a hyperbolic function of relative load. The minimum quick displacement required to reduce the force from the steady isometric value to half this value was about 1% of the fibre segment length.3. The relative force-velocity relation was independent of pCa in the range 5.0-6.75. Thus calcium ions appear to control the number of sites at which cross-bridges can be formed but have no significant effect on the kinetic properties of an individual bridge.4. Cross-bridges along the length of a myofilament appear to act independently of each other.5. The force-velocity relations reported for intact muscle fibres during relaxation, when compared with those of skinned fibres at different calcium levels, indicate that the time course of the fall in force after a stimulus is essentially that of calcium removal from the myofilaments.

Citing Articles

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When fibres go slack and cross bridges are free to run: a brilliant method to study kinetic properties of acto-myosin interaction.

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Determinants of force rise time during isometric contraction of frog muscle fibres.

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Inorganic phosphate affects the pCa-force relationship more than the pCa-ATPase by increasing the rate of dissociation of force generating cross-bridges in skinned fibers from both EDL and soleus muscles of the rat.

Kerrick W, Xu Y J Muscle Res Cell Motil. 2004; 25(2):107-17.

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Thin filament activation and unloaded shortening velocity of rabbit skinned muscle fibres.

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