Subgroups of Amino Acid Sequences in the Variable Regions of Immunoglobulin Heavy Chains

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1969 Nov 1

PMID 5264153

Citations 17

Authors

B A Cunningham

M N Pflumm

U Rutishauser

G M Edelman

Affiliations

Soon will be listed here.

Abstract

The amino acid sequence of the first 133 residues of the heavy (gamma) chain from a human gammaG immunoglobulin (He) has been determined. This gamma-chain is identical in Gm type to that of protein Eu, the complete sequence of which has been reported. Comparison of the two sequences substantiates the previous suggestion that there are subgroups of variable regions of heavy chains. The variable region of Eu has been assigned to subgroup I and that of He to subgroup II; on the other hand, the constant regions of the two proteins appear to be identical. Comparison of the sequence of the heavy chain of He with the heavy chain sequences determined in other laboratories suggests that the variable region of subgroup II is at least 118 residues long. The nature and distribution of amino acid variations in this heavy chain subgroup resemble those observed in light chain subgroups. These studies provide evidence that the translocation hypothesis applies to heavy as well as to light chains, viz., genes for variable regions (V) are somatically translocated to genes for constant regions (C) to form complete VC structural genes.

Citing Articles

Structural relationships among mouse and human immunoglobulin VH genes in the subgroup III.

Ollo R, Sikorav J, Rougeon F Nucleic Acids Res. 1983; 11(22):7887-97.

PMID: 6316276 PMC: 326547. DOI: 10.1093/nar/11.22.7887.

An analysis of the sequences of the variable regions of Bence Jones proteins and myeloma light chains and their implications for antibody complementarity.

Wu T, KABAT E J Exp Med. 1970; 132(2):211-50.

PMID: 5508247 PMC: 2138737. DOI: 10.1084/jem.132.2.211.

Localization of two additional hypervariable regions in immunoglobulin heavy chains.

Kehoe J, Capra J Proc Natl Acad Sci U S A. 1971; 68(9):2019-21.

PMID: 5289360 PMC: 389341. DOI: 10.1073/pnas.68.9.2019.

Heavy-chain variable regions in normal and pathological immunolobulins.

Wang A, Fudenberg H, Pink J Proc Natl Acad Sci U S A. 1971; 68(6):1143-6.

PMID: 5289298 PMC: 389138. DOI: 10.1073/pnas.68.6.1143.

Amino acid sequence of the light chain from a mouse myeloma protein with anti-hapten activity: evidence for a third type of light chain.

Schulenburg E, Simms E, Lynch R, Bradshaw R, Eisen H Proc Natl Acad Sci U S A. 1971; 68(11):2623-6.

PMID: 5288236 PMC: 389486. DOI: 10.1073/pnas.68.11.2623.

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