Nuclear Magnetic Resonance Spectroscopy: 13C Spectra of Some Common Nucleotides

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1970 Jan 1

PMID 5263750

Citations 12

Authors

D E Dorman

J D Roberts

Affiliations

Soon will be listed here.

Abstract

Natural-abundance (13)C nuclear magnetic resonance spectra of ten common nucleotides in neutral, aqueous solutions have been determined and interpreted. The spectra of two of these substances were also determined in acidic solutions, and several of the carbon chemical shifts were found to depend markedly on pH. Within the limited range of concentrations employed, there were observed no carbon chemical-shift changes which could be ascribed to base-stacking or base-pairing phenomena.

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References

Watson J, Crick F . Molecular structure of nucleic acids; a structure for deoxyribose nucleic acid. Nature. 1953; 171(4356):737-8. DOI: 10.1038/171737a0. View

Jones A, Winkley M, Grant D, ROBINS R . Carbon-13 nuclear magnetic resonance: naturally occurring nucleosides. Proc Natl Acad Sci U S A. 1970; 65(1):27-30. PMC: 286185. DOI: 10.1073/pnas.65.1.27. View

Weigert F, Roberts J . Nuclear magnetic resonance spectroscopy. Benzene-13C. J Am Chem Soc. 1967; 89(12):2967-9. DOI: 10.1021/ja00988a029. View

Chan S, Nelson J . Proton magnetic resonance studies of ribose dinucleoside monophoshates in aqueous solution. I. The nature of the base-stacking interaction in adenylyl 3'--5')adenosine. J Am Chem Soc. 1969; 91(1):168-83. DOI: 10.1021/ja01029a033. View

Weigert F, Jautelat M, Roberts J . Natural-abundance C nuclear magnetic resonance spectra of medium-molecular-weight organic compounds. Proc Natl Acad Sci U S A. 1968; 60(4):1152-5. PMC: 224894. DOI: 10.1073/pnas.60.4.1152. View