Kinetics of Induction and Purification of Chloramphenicol Acetyltransferase from Chloramphenicol-resistant Staphylococcus Aureus

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1969 Jun 1

PMID 4977987

Citations 14

Authors

E Winshell

W V Shaw

Affiliations

Soon will be listed here.

Abstract

Plasmid-mediated chloramphenicol resistance in Staphylococcus aureus has been shown to involve acetylation of chloramphenicol by an enzyme induced by growth in the presence of the antibiotic and certain analogues. Analysis of the kinetics of induction has been complicated by (i) the intrinsic inhibitory effects of chloramphenicol on induced enzyme synthesis and (ii) the rapid disappearance of inducer after synthesis of the acetylating enzyme. The compound related to d-threo chloramphenicol which lacks a C(3) hydroxyl substituent (3-deoxychloramphenicol) is a potent inducer of chloramphenicol acetyltransferase but is ineffective as an antibiotic and is not a substrate for the enzyme. The availability of such a "gratuitous" inducer has simplified an analysis of the kinetics of induction of chloramphenicol acetyltransferase. The enzyme from induced bacteria has been purified to homogeneity and has been compared with the analogous enzyme present in E. coli which harbors a resistance transfer factor with the chloramphenicol resistance determinant.

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References

Suzuki Y, Okamoto S . The enzymatic acetylation of chloramphenicol by the multiple drug-resistant Escherichia coli carrying R factor. J Biol Chem. 1967; 242(20):4722-30. View

CHABBERT Y, BAUDENS J, GERBAUD G . [VARIATIONS UNDER THE INFLUENCE OF ACRIFLAVIN AND TRANSDUCTION OF RESISTANCE TO KANAMYCIN AND CHLORAMPHENICOL IN STAPHYLOCOCCI]. Ann Inst Pasteur (Paris). 1964; 107:678-90. View

Jacob F, MONOD J . Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961; 3:318-56. DOI: 10.1016/s0022-2836(61)80072-7. View

Shaw W, Brodsky R . Characterization of chloramphenicol acetyltransferase from chloramphenicol-resistant Staphylococcus aureus. J Bacteriol. 1968; 95(1):28-36. PMC: 251967. DOI: 10.1128/jb.95.1.28-36.1968. View

Shaw W . The enzymatic acetylation of chloramphenicol by extracts of R factor-resistant Escherichia coli. J Biol Chem. 1967; 242(4):687-93. View

Davis B . DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964; 121:404-27. DOI: 10.1111/j.1749-6632.1964.tb14213.x. View

Okamoto S, Suzuki Y, Mise K, Nakaya R . Occurrence of chloramphenicol-acetylating enzymes in various gram-negative bacilli. J Bacteriol. 1967; 94(5):1616-22. PMC: 276870. DOI: 10.1128/jb.94.5.1616-1622.1967. View

Nathans D . INHIBITION OF PROTEIN SYNTHESIS BY PUROMYCIN. Fed Proc. 1964; 23:984-9. View

Suzuki Y, Okamoto S, Kono M . Basis of chloramphenicol resistance in naturally isolated resistant staphylococci. J Bacteriol. 1966; 92(3):798-9. PMC: 276329. DOI: 10.1128/jb.92.3.798-799.1966. View

10.

Cohen S, SWEENEY H, Leitner F . TEMPERATURE-SENSITIVE REPRESSION OF STAPHYLOCOCCAL PENICILLINASE. Science. 1965; 149(3686):877-9. DOI: 10.1126/science.149.3686.877. View

11.

Novick R . Penicillinase plasmids of Staphylococcus aureus. Fed Proc. 1967; 26(1):29-38. View

12.

Shaw W . Enzymatic chlorampheicol acetylation and R factor induced antibiotic resistance in Enterobacteriaceae. Antimicrob Agents Chemother (Bethesda). 1966; 6:221-6. View

13.

Kono M, Ogawa K, Mitsuhashi S . Drug resistance of staphylococci. VI. Genetic determinant for chloramphenicol resistance. J Bacteriol. 1968; 95(3):886-92. PMC: 252106. DOI: 10.1128/jb.95.3.886-892.1968. View

14.

Novick R, Richmond M . NATURE AND INTERACTIONS OF THE GENETIC ELEMENTS GOVERNING PENICILLINASE SYNTHESIS IN STAPHYLOCOCCUS AUREUS. J Bacteriol. 1965; 90:467-80. PMC: 315668. DOI: 10.1128/jb.90.2.467-480.1965. View

15.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

16.

Shaw W, Brodsky R . Chloramphenicol resistance by enzymatic acetylation: comparative aspects. Antimicrob Agents Chemother (Bethesda). 1967; 7:257-63. View

17.

Alpers D, Tomkins G . THE ORDER OF INDUCTION AND DEINDUCTION OF THE ENZYMES OF THE LACTOSE OPERON IN E. COLI. Proc Natl Acad Sci U S A. 1965; 53:797-802. PMC: 221069. DOI: 10.1073/pnas.53.4.797. View

18.

Brock T . CHLORAMPHENICOL. Bacteriol Rev. 1961; 25(1):32-48. PMC: 441072. DOI: 10.1128/br.25.1.32-48.1961. View

19.

Sabath L, Gerstein D, LODER P, FINLAND M . Independent segregation of chloramphenicol resistance in Staphylococcus aureus. Antimicrob Agents Chemother (Bethesda). 1967; 7:264-70. View

20.

Miyamura S . INACTIVATION OF CHLORAMPHENICOL BY CHLORAMPHENICOL-RESISTANT BACTERIA. J Pharm Sci. 1964; 53:604-7. DOI: 10.1002/jps.2600530606. View