Hybridization of Variants of Chloramphenicol Acetyltransferase Specified by Fi + and Fi - R Factors

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1972 Oct 1

PMID 4628098

Citations 8

Authors

W V Shaw

L C Sands

N Datta

Affiliations

Soon will be listed here.

Abstract

An fi(-) type of R factor for transferable chloramphenicol resistance carries the structural gene for a novel species of chloramphenicol acetyltransferase (EC 2.3.1.99). The enzyme associated with the fi(-) plasmid is distinct from that described for fi(+) R factors. The fi(+)- and fi(-)-related variants of chloramphenicol acetyltransferase were hybridized in vivo and in vitro. Both techniques yielded only a single symmetrical heteromeric species rather than the three (A(3)B(1), A(2)B(2), and A(1)B(3)) hybrids predicted from the tetrameric (identical monomers) structure of chloramphenicol acetyltransferase.

Citing Articles

Differentiation of IncL and IncM Plasmids Associated with the Spread of Clinically Relevant Antimicrobial Resistance.

Carattoli A, Seiffert S, Schwendener S, Perreten V, Endimiani A PLoS One. 2015; 10(5):e0123063.

PMID: 25933288 PMC: 4416936. DOI: 10.1371/journal.pone.0123063.

The use of naturally occurring hybrid variants of chloramphenicol acetyltransferase to investigate subunit contacts.

Packman L, Shaw W Biochem J. 1981; 193(2):541-52.

PMID: 7030311 PMC: 1162635. DOI: 10.1042/bj1930541.

Identification of "buried" lysine residues in two variants of chloramphenicol acetyltransferase specified by R-factors.

Packman L, Shaw W Biochem J. 1981; 193(2):525-39.

PMID: 6796049 PMC: 1162634. DOI: 10.1042/bj1930525.

Mechanism of chloramphenicol resistance in staphylococci: characterization and hybridization of variants of chloramphenicol acetyltransferase.

Sands L, Shaw W Antimicrob Agents Chemother. 1973; 3(2):299-305.

PMID: 4790593 PMC: 444403. DOI: 10.1128/AAC.3.2.299.

Chloramphenicol acetyltransferases specified by fi minus R factors.

Foster T, Shaw W Antimicrob Agents Chemother. 1973; 3(1):99-104.

PMID: 4597710 PMC: 444367. DOI: 10.1128/AAC.3.1.99.

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