Use of Hybrid Antibody with Anti-gamma-G and Anti-ferritin Specificities in Locating Cell Surface Antigens by Electron Microscopy

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1968 Dec 1

PMID 4972125

Citations 32

Authors

U Hammerling

T Aoki

E de Harven

E A Boyse

L J Old

Affiliations

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Abstract

Hybrid antibody [F(ab')(2)] with dual specificity for mouse gammaG and ferritin was prepared from the corresponding rabbit antisera, providing a precise reagent for locating mouse gammaG on cell surfaces. Viable cells were exposed successively to (a) mouse antibody to a cell surface antigen, (b) the rabbit hybrid antibody, and (c) ferritin, before preparation for electron microscopy. This method of See PDF for Structure. labeling is sensitive and specific and clearly lends itself to the introduction of visual markers other than ferritin. Other advantages are uniformity of labeling, ease of purification of the reagent, and circumvention of the many drawbacks arising from coupling ferritin to antibody chemically.

Citing Articles

Symmetry breaking: bispecific antibodies, the beginnings, and 50 years on.

Riethmuller G Cancer Immun. 2012; 12:12.

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[Membrane asectsin immunology].

Fischer H, Rude E, Sellin D Naturwissenschaften. 1970; 57(11):507-13.

PMID: 5491726 DOI: 10.1007/BF00625316.

Antigenic structure of cell surfaces. An immunoferritin study of the occurrence and topography of H-2' theta, and TL alloantigens on mouse cells.

Aoki T, Hammerling U, de Harven E, Boyse E, Old L J Exp Med. 1969; 130(5):979-1001.

PMID: 5347699 PMC: 2180493. DOI: 10.1084/jem.130.5.979.

G (Gross) and H-2 cell-surface antigens: location on Gross leukemia cells by electron microscopy with visually labeled antibody.

Aoki T, Boyse E, Old L, de Harven E, Hammerling U, Wood H Proc Natl Acad Sci U S A. 1970; 65(3):569-76.

PMID: 5267138 PMC: 282945. DOI: 10.1073/pnas.65.3.569.

Ultrastructural localization of human HL-A membrane antigens by use of hybrid antibodies.

Neauport-Sautes C, Silvestre D, Niccolai M, KOURILSKY F, LEVY J Immunology. 1972; 22(5):833-45.

PMID: 5063188 PMC: 1407859.

References

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