Topographical Location of H-Y Antigen on Mouse Spermatozoa by Immunoelectronmicroscopy

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1973 May 1

PMID 4576022

Citations 23

Authors

G C Koo

C W Stackpole

E A Boyse

U Hammerling

M P LARDIS

Affiliations

Soon will be listed here.

Abstract

H-Y (male) antigen was visually located on mouse sperm by electron microscopy, by use of the indirect hybrid antibody method and tobacco mosaic virus as the visual marker. Labeling was achieved by centrifugation of the sperm through a discontinuous gradient consisting of alternating layers of immune reagents and wash solutions. Treated sperm were examined topographically by preparation of platinum-carbon replicas. Antigen was located mainly on the acrosomal cap of the sperm head.

Citing Articles

Radioimmunoassay reveals no anti-male specificity in putative H-Y antisera.

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Rogalski A, Bouck G J Cell Biol. 1982; 93(3):758-66.

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Application of monoclonal anti-HY antibody for human H-Y typing.

Koo G, Tada N, Chaganti R, Hammerling U Hum Genet. 1981; 57(1):64-7.

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Characterization and localization of a flagellar-specific membrane glycoprotein in Euglena.

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Inherited XX sex reversal in the cocker spaniel dog.

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