Host Effect on Arbovirus Replication: Appearance of Defective Interfering Particles in Murine Cells

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1973 Dec 1

PMID 4796899

Citations 8

Authors

J G LEVIN

J M Ramseur

P M Grimley

Affiliations

Soon will be listed here.

Abstract

Serial passage of Semliki Forest virus (SFV) in chicken embryo cells had little effect on SFV yield; however, high multiplicity infection of murine cells with one of the late passage pools (passage 9 SFV) resulted in a virus yield 10- to 20-fold lower than that obtained with earlier passage virus and 80-fold lower than the corresponding yield in chicken cells. This effect was accompanied by a striking decrease in the levels of 42S and 26S RNA and by increased proportions of a small single-stranded viral RNA (molecular weight, 9 x 10(5)) and of a low-molecular-weight replicative form. There was also a reduction in the number of specific membranous structures previously associated with the group A arbovirus replication complex. These results suggested that passage 9 SFV contained defective interfering particles which were detected more readily after one passage in a murine indicator host cell. Identical results were obtained with two different murine cell lines: one a leukemia virus-free clone of AKR cells and the other JLS-V9 cells chronically infected with Rauscher leukemia virus. Host production of RNA tumor virus particles apparently did not affect arbovirus replication.

Citing Articles

Shared sequence characteristics identified in non-canonical rearrangements of HSV-1 genomes.

Shitrit A, Nisnevich V, Rozenshtein N, Kobo H, Phan H, Tay S J Virol. 2023; 97(12):e0095523.

PMID: 37991369 PMC: 10734421. DOI: 10.1128/jvi.00955-23.

Laboratory Validation of a Real-Time RT-PCR Assay for the Detection of Jamestown Canyon Virus.

Hughes H, Kenney J, Russell B, Lambert A Pathogens. 2022; 11(5).

PMID: 35631056 PMC: 9146205. DOI: 10.3390/pathogens11050536.

Homologous interference induced by Sindbis virus.

Johnston R, Wan K, Bose H J Virol. 1974; 14(5):1076-82.

PMID: 4473566 PMC: 355622. DOI: 10.1128/JVI.14.5.1076-1082.1974.

Limited infection of mouse brain cell cultures with Semliki forest virus.

SEAMER J Br J Exp Pathol. 1974; 55(6):606-14.

PMID: 4447795 PMC: 2072733.

Deficiency of 60 to 70S RNA in murine leukemia virus particles assembled in cells treated with actinomycin D.

LEVIN J, Grimley P, Ramseur J, Berezesky I J Virol. 1974; 14(1):152-61.

PMID: 4134468 PMC: 355489. DOI: 10.1128/JVI.14.1.152-161.1974.

References

Friedman R, Levy H, Carter W . Replication of semliki forest virus: three forms of viral RNA produced during infection. Proc Natl Acad Sci U S A. 1966; 56(2):440-6. PMC: 224391. DOI: 10.1073/pnas.56.2.440. View

Sonnabend J, Martin E, MECS E . Viral specific RNAs in infected cells. Nature. 1967; 213(5074):365-7. DOI: 10.1038/213365a0. View

WRIGHT B, OBrien P, Shibley G, MAYYASI S, Lasfargues J . Infection of an established mouse bone marrow cell line (JLS-V9) with Rauscher and Moloney murine leukemia viruses. Cancer Res. 1967; 27(9):1672-7. View

Friedman R . Structural and nonstructural proteins of an arbovirus. J Virol. 1968; 2(10):1076-80. PMC: 375438. DOI: 10.1128/JVI.2.10.1076-1080.1968. View

Grimley P, Berezesky I, Friedman R . Cytoplasmic structures associated with an arbovirus infection: loci of viral ribonucleic acid synthesis. J Virol. 1968; 2(11):1326-38. PMC: 375472. DOI: 10.1128/JVI.2.11.1326-1338.1968. View

Stampfer M, Baltimore D, Huang A . Ribonucleic acid synthesis of vesicular stomatitis virus. I. Species of ribonucleic acid found in Chinese hamster ovary cells infected with plaque-forming and defective particles. J Virol. 1969; 4(2):154-61. PMC: 375850. DOI: 10.1128/JVI.4.2.154-161.1969. View

Choppin P . Replication of influenza virus in a continuous cell line: high yield of infective virus from cells inoculated at high multiplicity. Virology. 1969; 39(1):130-4. DOI: 10.1016/0042-6822(69)90354-7. View

Huang A, Baltimore D . Defective viral particles and viral disease processes. Nature. 1970; 226(5243):325-7. DOI: 10.1038/226325a0. View

Friedman R . Protein synthesis directed by an arbovirus. J Virol. 1968; 2(1):26-32. PMC: 375574. DOI: 10.1128/JVI.2.1.26-32.1968. View

10.

Martin E, Sonnabend J . Ribonucleic acid polymerase catalyzing synthesis of double-stranded arbovirus ribonucleic acid. J Virol. 1967; 1(1):97-109. PMC: 375509. DOI: 10.1128/JVI.1.1.97-109.1967. View

11.

Friedman R, Berezesky I . Cytoplasmic fractions associated with Semliki Forest virus ribonucleic acid replication. J Virol. 1967; 1(2):374-83. PMC: 375236. DOI: 10.1128/JVI.1.2.374-383.1967. View

12.

Kingsbury D, Portner A . On the genesis of incomplete Sendai virions. Virology. 1970; 42(4):872-9. DOI: 10.1016/0042-6822(70)90336-3. View

13.

LEVIN J, Friedman R . Analysis of arbovirus ribonucleic acid forms by polyacrylamide gel electrophoresis. J Virol. 1971; 7(4):504-14. PMC: 356151. DOI: 10.1128/JVI.7.4.504-514.1971. View

14.

Rowe W, Hartley J, Lander M, PUGH W, Teich N . Noninfectious AKR mouse embryo cell lines in which each cell has the capacity to be activated to produce infectious murine leukemia virus. Virology. 1971; 46(3):866-76. DOI: 10.1016/0042-6822(71)90087-0. View

15.

Schlesinger S, Schlesinger M, BURGE B . Defective virus particles from Sindbis virus. Virology. 1972; 48(2):615-7. DOI: 10.1016/0042-6822(72)90076-1. View

16.

Grimley P, LEVIN J, Berezesky I, Friedman R . Specific membranous structures associated with the replication of group A arboviruses. J Virol. 1972; 10(3):492-503. PMC: 356491. DOI: 10.1128/JVI.10.3.492-503.1972. View

17.

Friedman R, LEVIN J, Grimley P, Berezesky I . Membrane-associated replication complex in arbovirus infection. J Virol. 1972; 10(3):504-15. PMC: 356492. DOI: 10.1128/JVI.10.3.504-515.1972. View

18.

Shenk T, Stollar V . Viral RNA species in BHK-21 cells infected with Sindbis virus serially passaged at high multiplicity of infection. Biochem Biophys Res Commun. 1972; 49(1):60-7. DOI: 10.1016/0006-291x(72)90009-5. View

19.

Perrault J, Holland J . Variability of vesicular stomatitis virus autointerference with different host cells and virus serotypes. Virology. 1972; 50(1):148-58. DOI: 10.1016/0042-6822(72)90355-8. View

20.

Eaton B, Faulkner P . Altered pattern of viral RNA synthesis in cells infected with standard and defective Sindbis virus. Virology. 1973; 51(1):85-93. DOI: 10.1016/0042-6822(73)90368-1. View