Mumps Class-specific Immunoglobulins in Radioimmunoassay and Conventional Serology

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1973 Mar 1

PMID 4713690

Citations 14

Authors

H Daugharty

D T Warfield

W D Hemingway

H L CASEY

Affiliations

Soon will be listed here.

Abstract

Antibodies against mumps virus have been studied by using immunoglobulin class-specific indicators labeled with (125)I in the radioimmunoassay (RIA) procedure. The immunoglobulins in paired acute and convalescent sera were allowed to react with mumps virus in a solid-phase RIA system. Class-specific immunoglobulin indicators (anti-immunoglobulin M [IgM] and anti-immunoglobulin G [IgG]) labeled with (125)I revealed that immunoglobulins of early antisera were preponderantly IgM, whereas immunoglobulins of late antisera were predominantly IgG. These indicators detected antibodies of the early (IgM) and late (IgG) phases of the immune response. These findings are consistent with the classical temporal order of appearance of 19s (IgM) and 7s (IgG) globulins. Specificity of these indicators for reacting with fractionated 7s and 19s globulins is also presented. Mumps virus RIA obtained with anti-IgG correlated well with conventional serological data obtained by neutralization and hemagglutination inhibition, but most strongly with complement-fixation data. In addition, antibody bound by solid phase was capable of distinguishing between related antigens of the myxovirus group.

Citing Articles

Enzyme-linked immunosorbent assay (ELISA) for detection of specific IgA antibodies to mumps virus.

Halevy B, Sarov I J Clin Pathol. 1982; 35(10):1129-33.

PMID: 7130422 PMC: 497896. DOI: 10.1136/jcp.35.10.1129.

Chemiluminescence-linked immunoassay for detection of mumps virus antibodies.

Konishi E, Iwasa S, Kondo K, Hori M J Clin Microbiol. 1980; 12(2):140-3.

PMID: 7014604 PMC: 273543. DOI: 10.1128/jcm.12.2.140-143.1980.

Sensitive hemagglutination inhibition test for mumps antibody.

Albrecht P, Klutch M J Clin Microbiol. 1981; 13(5):870-6.

PMID: 6972385 PMC: 273906. DOI: 10.1128/jcm.13.5.870-876.1981.

Comparison of nonspecific reactivity in indirect and reverse immunoassays for measles and mumps immunoglobulin M antibodies.

Tuokko H J Clin Microbiol. 1984; 20(5):972-6.

PMID: 6392329 PMC: 271486. DOI: 10.1128/jcm.20.5.972-976.1984.

Detection of IgM antibodies to measles virus by enzyme-immunoassay.

Tuokko H, Salmi A Med Microbiol Immunol. 1983; 171(4):187-98.

PMID: 6343817 DOI: 10.1007/BF02123492.

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