Solid-phase Radioimmunoassay of Total and Influenza-specific Immunoglobulin G

Overview

Journal Appl Microbiol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1972 Feb 1

PMID 5062884

Citations 16

Authors

H Daugharty

D T Warfield

M L Davis

Affiliations

Soon will be listed here.

Abstract

An antigen-antibody system of polystyrene tubes coated with immunoglobulin antibody was used for quantitating immunoglobulins. A similar radioimmunoassay method was adapted for a viral antigen-antibody system. The viral system can be used for quantitating viruses and for measuring virus-specific antibodies by reacting with (125)iodine-labeled anti-immunoglobulin G (IgG). Optimal conditions for coating the solid phase, specificity of the immune reaction, and other kinetics and sensitivities of the assay method were investigated. Comparison of direct and indirect methods of assaying for immunoglobulins or viral antibody indicates that the indirect method is more sensitive and can quantitate a minimum of 0.037 mug of IgG per ml. Results of solid-phase radioimmunoassay for influenza antibody correlate well with hemagglutinin antibody titers but not with complement-fixing antibody titers. Radioimmunoassay results for influenza antibody by solid phase are likewise in agreement with results by the carrier precipitate radioimmunoassay method. The simplicity, reproducibility, and versatility of the solid-phase procedure make it diagnostically useful.

Citing Articles

Rapid bead counting and dispensing device for radioimmunoassays and enzyme-linked immunosorbent assays.

Hui G J Clin Microbiol. 1981; 13(4):801-4.

PMID: 7014629 PMC: 273884. DOI: 10.1128/jcm.13.4.801-804.1981.

Labeling of antibodies with 3H-acetate.

Kalmakoff J, Parkinson A Appl Microbiol. 1973; 26(4):624-6.

PMID: 4796168 PMC: 379862. DOI: 10.1128/am.26.4.624-626.1973.

Nonspecific adsorption of proteins to microplates.

Inouye S Appl Microbiol. 1973; 25(2):279-83.

PMID: 4735108 PMC: 380787. DOI: 10.1128/am.25.2.279-283.1973.

Mumps class-specific immunoglobulins in radioimmunoassay and conventional serology.

Daugharty H, Warfield D, Hemingway W, CASEY H Infect Immun. 1973; 7(3):380-5.

PMID: 4713690 PMC: 422688. DOI: 10.1128/iai.7.3.380-385.1973.

Comparison of direct and indirect solid-phase microradioimmunoassays for the detection of viral antigens and antiviral antibody.

Rosenthal J, Hayashi K, Notkins A Appl Microbiol. 1973; 25(4):567-73.

PMID: 4349247 PMC: 380863. DOI: 10.1128/am.25.4.567-573.1973.

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