Detection of IgM Antibodies to Measles Virus by Enzyme-immunoassay

Overview

Journal Med Microbiol Immunol

Specialty Allergy & Immunology

Date 1983 Jan 1

PMID 6343817

Citations 8

Authors

H Tuokko

A Salmi

Affiliations

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Abstract

A method of solid-phase enzyme-immunoassay (EIA) with horseradish-peroxidase-conjugated anti-mu-globulin was used to determine IgM antibodies to measles virus in human sera. Antigens prepared from measles-infected and noninfected Vero cells passively adsorbed to polystyrene cuvettes were both important for the tests, because sera from many patients convalescing from viral infections and sera from rheumatoid arthritis patients were able to bind to control antigen. Sera from measles patients, patients with other viral infections, rheumatoid arthritis (RA) patients, and from blood donors were tested in a dilution of 1:200. Almost all of the acute-phase measles sera (37/38) were positive. The first measles-IgM-negative specimen was found 9 weeks after the onset of rash. Seven percent (15/213) of patients with other viral infections gave positive results in these IgM tests. False-positive reactions were also found in 33 of 51 (65%) sera from RA patients. This nonspecific binding could be abolished by absorptions with latex-IgG particles. This treatment did not have any effect on specific IgM. The IgM-EIA test developed for measles virus antibodies, which requires only a single serum specimen, appears to be a useful diagnostic method for routine virus laboratories.

Citing Articles

Evaluation of hemagglutinin protein-specific immunoglobulin M for diagnosis of measles by an enzyme-linked immunosorbent assay based on recombinant protein produced in a high-efficiency mammalian expression system.

Bouche F, Brons N, Houard S, Schneider F, Muller C J Clin Microbiol. 1998; 36(12):3509-13.

PMID: 9817863 PMC: 105230. DOI: 10.1128/JCM.36.12.3509-3513.1998.

Comparison of nonspecific reactivity in indirect and reverse immunoassays for measles and mumps immunoglobulin M antibodies.

Tuokko H J Clin Microbiol. 1984; 20(5):972-6.

PMID: 6392329 PMC: 271486. DOI: 10.1128/jcm.20.5.972-976.1984.

Specific immunoglobulin M enzyme-linked immunosorbent assay for confirming the diagnosis of measles.

Lievens A, Brunell P J Clin Microbiol. 1986; 24(3):391-4.

PMID: 3760134 PMC: 268921. DOI: 10.1128/jcm.24.3.391-394.1986.

Assay of measles virus IgM and IgG class antibodies by use of peroxidase-labelled viral antigens.

Salonen J, Vainionpaa R, Halonen P Arch Virol. 1986; 91(1-2):93-106.

PMID: 3530194 DOI: 10.1007/BF01316731.

Viral infections and recurrences of febrile convulsions.

Rantala H, Uhari M, Tuokko H J Pediatr. 1990; 116(2):195-9.

PMID: 2299489 PMC: 7172996. DOI: 10.1016/s0022-3476(05)82874-4.

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