The Electrical Activity of Canine Cardiac Purkinje Fibers in Sodium-free, Calcium-rich Solutions

Overview

Journal J Gen Physiol

Specialty Physiology

Date 1973 Jun 1

PMID 4708407

Citations 13

Authors

R S Aronson

P F Cranefield

Affiliations

Soon will be listed here.

Abstract

Propagated action potentials can be obtained in canine cardiac Purkinje fibers exposed to Na-free solutions containing no inorganic cation other than Ca and K. Essentially similar action potentials are obtained if Na is replaced by tetraethylammonium (TEA), tetramethylammonium (TMA), or choline. In a solution containing 128 mM TEA and 16.2 mM Ca the characteristics of these electrical responses were: maximum diastolic potential, -59 +/- 3.3 mV; overshoot, 20 +/- 6.8 mV; maximum upstroke velocity, 3.7 +/- 2.3 V/s; conduction velocity, 0.1 m/s; and action potential duration, 360 +/- 45 ms. The magnitude of the overshoot varied with log Ca(o) with a slope of about 30 mV/10-fold concentration change. The upstroke velocity was an approximately linear function of Ca(o). The active response was greatly diminished or abolished by Mn and D-600 but was unaffected by tetrodotoxin. These Ca-dependent responses appeared in a region of transmembrane potential (about -50 mV) at which the rapid Na-dependent upstroke is abolished even when Na is present.

Citing Articles

Calcium-activated transient outward current in calf cardiac Purkinje fibres.

Siegelbaum S, Tsien R J Physiol. 1980; 299:485-506.

PMID: 6770079 PMC: 1279238. DOI: 10.1113/jphysiol.1980.sp013138.

Effect of intracellular tetraethylammonium ion on action potential in the guinea-pig's myocardium.

Ochi R, Nishiye H Pflugers Arch. 1974; 348(4):305-16.

PMID: 4857772 DOI: 10.1007/BF00589220.

The effect of resting potential on the electrical activity of canine cardiac Purkinje fibers exposed to Na-free solution or to ouabain.

Aronson R, Cranefield P Pflugers Arch. 1974; 347(2):101-16.

PMID: 4856534 DOI: 10.1007/BF00592392.

Effect on membrane potential and electrical activity of adding sodium to sodium-depleted cardiac purkinje fibers.

Wiggins J, Cranefield P J Gen Physiol. 1974; 64(4):473-93.

PMID: 4418558 PMC: 2226156. DOI: 10.1085/jgp.64.4.473.

Reconstruction of the electrical activity of cardiac Purkinje fibres.

McAllister R, Noble D, Tsien R J Physiol. 1975; 251(1):1-59.

PMID: 1185607 PMC: 1348375. DOI: 10.1113/jphysiol.1975.sp011080.

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