Cell-free Synthesis of a Specific Lipoprotein of the Escherichia Coli Outer Membrane Directed by Purified Messenger RNA

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1974 Oct 1

PMID 4610570

Citations 20

Authors

A Hirashima

S Wang

M Inouye

Affiliations

Soon will be listed here.

Abstract

A specific lipoprotein of the E. coli outer membrane has been synthesized in a cell-free system directed by purified messenger RNA. The mRNA for the lipoprotein was purified as 7S RNA about 250-fold from exponentially growing cells. Protein synthesis of the cell-free system was totally dependent upon the addition of the purified mRNA. The product of the cell-free system was identified as the specific lipoprotein by immunoprecipitation and by peptide mapping.

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