Article: Restriction enzyme cleavage sites surrounding the structural gene for the lipoprotein of the Escherichia coli outer membrane

The purified messenger ribonucleic acid (mRNA) for the lipoprotein of the Escherichia coli outer membrane was hybridized with fragments obtained by digestion of E. coli chromosomal deoxyribonucleic acid (DNA) with eight different restriction enzymes. For each restriction enzyme digestion, one specific fragment separated by agarose gel electrophoresis was found to hybridize with the lipoprotein mRNA. From the analysis of restriction fragments generated by double digestions with various combinations of restriction enzymes, cleavage sites for the restriction enzymes near the locus of the lipoprotein structural gene (lpp) were mapped. No restriction fragments of DNA from the E. coli lpp-2 mutant hybridized with the lipoprotein mRNA, confirming that the mutant has a deletion mutation in the vicinity of the lpp gene.

Citing Articles

A physical map of the Myxococcus xanthus chromosome.

He Q, Chen H, Kuspa A, Cheng Y, Kaiser D, Shimkets L Proc Natl Acad Sci U S A. 1994; 91(20):9584-7.

PMID: 7937810 PMC: 44857. DOI: 10.1073/pnas.91.20.9584.

Analysis of Tn916-induced mutants of Clostridium acetobutylicum altered in solventogenesis and sporulation.

Mattsson D, Rogers P J Ind Microbiol. 1994; 13(4):258-68.

PMID: 7765050 DOI: 10.1007/BF01569758.

Bacillus licheniformis penicillinase synthesized in Escherichia coli contains covalently linked fatty acid and glyceride.

Lai J, Sarvas M, Brammar W, Neugebauer K, Wu H Proc Natl Acad Sci U S A. 1981; 78(6):3506-10.

PMID: 7022452 PMC: 319598. DOI: 10.1073/pnas.78.6.3506.

Expression of the Serratia marcescens lipoproteins gene in Escherichia coli.

Lee N, Nakamura K, Inouye M J Bacteriol. 1981; 146(3):861-6.

PMID: 7016834 PMC: 216937. DOI: 10.1128/jb.146.3.861-866.1981.

DNA sequence of the Serratia marcescens lipoprotein gene.

Nakamura K, Inouye M Proc Natl Acad Sci U S A. 1980; 77(3):1369-73.

PMID: 6990409 PMC: 348496. DOI: 10.1073/pnas.77.3.1369.

References

1.

Blattner F, Williams B, Blechl A, Faber H, Furlong L, Grunwald D . Charon phages: safer derivatives of bacteriophage lambda for DNA cloning. Science. 1977; 196(4286):161-9. DOI: 10.1126/science.847462. View

2.

Nakamura K, Pirtle R, Inouye M . Homology of the gene coding for outer membrane lipoprotein within various Gram-negative bacteria. J Bacteriol. 1979; 137(1):595-604. PMC: 218487. DOI: 10.1128/jb.137.1.595-604.1979. View

3.

DiRienzo J, Nakamura K, Inouye M . The outer membrane proteins of Gram-negative bacteria: biosynthesis, assembly, and functions. Annu Rev Biochem. 1978; 47:481-532. DOI: 10.1146/annurev.bi.47.070178.002405. View

4.

Pirtle R, Pirtle I, Inouye M . Homologous nucleotide sequences between prokaryotic and eukaryotic mRNAs: the 5'-end sequence of the mRNA of the lipoprotein of the Escherichia coli outer membrane. Proc Natl Acad Sci U S A. 1978; 75(5):2190-4. PMC: 392517. DOI: 10.1073/pnas.75.5.2190. View

5.

Hirota Y, Suzuki H, Nishimura Y, Yasuda S . On the process of cellular division in Escherichia coli: a mutant of E. coli lacking a murein-lipoprotein. Proc Natl Acad Sci U S A. 1977; 74(4):1417-20. PMC: 430781. DOI: 10.1073/pnas.74.4.1417. View

6.

Takeishi K, Yasumura M, Pirtle R, Inouye M . Isolation and identification of the messenger ribonucleic acid for a structural lipoprotein of the Escherichia coli outer membrane. J Biol Chem. 1976; 251(20):6259-66. View

7.

Bachmann B, Low K, Taylor A . Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev. 1976; 40(1):116-67. PMC: 413944. DOI: 10.1128/br.40.1.116-167.1976. View

8.

Suzuki H, Nishimura Y, Iketani H, Campisi J, Hirashima A . Novel mutation that causes a structural change in a lipoprotein in the outer membrane of Escherichia coli. J Bacteriol. 1976; 127(3):1494-1501. PMC: 232945. DOI: 10.1128/jb.127.3.1494-1501.1976. View

9.

Hirashima A, Wang S, Inouye M . Cell-free synthesis of a specific lipoprotein of the Escherichia coli outer membrane directed by purified messenger RNA. Proc Natl Acad Sci U S A. 1974; 71(10):4149-53. PMC: 434347. DOI: 10.1073/pnas.71.10.4149. View

10.

Thomas M, Davis R . Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease. J Mol Biol. 1975; 91(3):315-28. DOI: 10.1016/0022-2836(75)90383-6. View

11.

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

Restriction Enzyme Cleavage Sites Surrounding the Structural Gene for the Lipoprotein of the Escherichia Coli Outer Membrane