MRNA-dependent in Vitro Synthesis of Ribosomal Proteins L12 and L10 and Elongation Factor Tu

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1977 Dec 1

PMID 341155

Citations 4

Authors

F Chu

P Caldwell

H Weissbach

N Brot

Affiliations

Soon will be listed here.

Abstract

RNA extracted from growing Escherichia coli can direct the in vitro synthesis of ribosomal proteins L12 and L10 and elongation factor Tu when an E. coli system is used. The synthesized L12 can be bound to L12-depleted ribosomes and the synthesized elongation factor Tu can form complexes with both elongation factor Ts and GDP. Guanosine 5'-diphosphate 3'-diphosphate has no effect on the synthesis of these proteins from an RNA template but inhibits their synthesis when a DNA template is used.

Citing Articles

Autogenous control of Escherichia coli ribosomal protein L10 synthesis in vitro.

Brot N, Caldwell P, Weissbach H Proc Natl Acad Sci U S A. 1980; 77(5):2592-5.

PMID: 6994102 PMC: 349448. DOI: 10.1073/pnas.77.5.2592.

The search for guanosine tetraphosphate (ppGpp) and other unusual nucleotides in eucaryotes.

Silverman R, Atherly A Microbiol Rev. 1979; 43(1):27-41.

PMID: 379576 PMC: 281460. DOI: 10.1128/mr.43.1.27-41.1979.

In vitro regulation of DNA-dependent synthesis of Escherichia coli ribosomal protein L12.

Goldberg G, Caldwell P, Weissbach H, Brot N Proc Natl Acad Sci U S A. 1979; 76(4):1716-20.

PMID: 287012 PMC: 383461. DOI: 10.1073/pnas.76.4.1716.

Eukaryotic ribosomal proteins stimulate Escherichia coli stringent factor to synthesize guanosine 5'-diphosphate, 3'-diphosphate (ppGpp) and guanosine 5'-triphosphate, 3'-diphosphate (ppGpp).

Martini O, Richter D Mol Gen Genet. 1978; 166(3):291-7.

PMID: 216901 DOI: 10.1007/BF00267621.

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