Recircularization and Autonomous Replication of a Sheared R-factor DNA Segment in Escherichia Coli Transformants

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1973 May 1

PMID 4576014

Citations 93

Authors

S N Cohen

A C Chang

Affiliations

Soon will be listed here.

Abstract

Controlled shearing of R-factor DNA leads to formation of fragments carrying an antibiotic resistance gene present on, but not expressed by, the intact R-factor. Transformation of CaCl(2)-treated E. coli by such fragments yields an autonomously replicating tetracycline-resistance plasmid (Tc6-5) that contains only a small fraction of the genome of the parent R-factor, and lacks both its fertility functions and its other drug-resistance determinants. Although the Tc6-5 plasmid is not self-transmissible, it can interact and/or recombine with conjugally-proficient plasmids that promote its transfer to other bacteria.

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