Acid-soluble Breakdown of Homologous Deoxyribbonucleic Acid Adsorbed by Haemophilus Influenzae: Its Biological Significance

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1974 Nov 1

PMID 4549063

Citations 7

Authors

J H STUY

Affiliations

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Abstract

Competent bacteria of Haemophilus influenzae strain Rd were exposed to various kinds of radioactive deoxyribonucleic acid (DNA) for short periods of time and at relatively low temperature. The fate of phage HP1 DNA was studied most extensively. Adsorbed DNA was partially acid solubilized by lysogens and by nonlysogens with very similar kinetics. The biological activity of the DNA decreased extensively in both lysogenic and nonlysogenic recipients. 2,4-Dinitrophenol had no effect on the acid solubilization but largely abolished the biological inactivation. Inactivation kinetics for three different markers and for the triple combination were roughly the same. The presence of 2,4-dinitrophenol in the medium, or the HP1 prophage in the chromosome, did not alter this observation. This suggests that acid solubilization involves the destruction of whole DNA molecules. In view of the absence of DNA homology between phage and host, it is concluded that acid-soluble breakdown of adsorbed transforming DNA is not an integral part of the donor DNA integration process. Behavior of mutant bacteria indicates that neither exonuclease III nor exonuclease V is involved.

Citing Articles

Transformation in Haemophilus: a problem in membrane biology.

Kahn M, Smith H J Membr Biol. 1984; 81(2):89-103.

PMID: 6387128 DOI: 10.1007/BF01868974.

Directional transport and integration of donor DNA in Haemophilus influenzae transformation.

BARANY F, Kahn M, Smith H Proc Natl Acad Sci U S A. 1983; 80(23):7274-8.

PMID: 6316352 PMC: 390037. DOI: 10.1073/pnas.80.23.7274.

Single-stranded regions in transforming deoxyribonucleic acid after uptake by competent Haemophilus influenzae.

Sedgwick B, Setlow J J Bacteriol. 1976; 125(2):588-94.

PMID: 1081987 PMC: 236119. DOI: 10.1128/jb.125.2.588-594.1976.

Fate of transforming bacteriophage HP1 deoxyribonucleic acid in Haemophilus influenzae lysogens.

STUY J J Bacteriol. 1975; 122(3):1038-44.

PMID: 1080148 PMC: 246157. DOI: 10.1128/jb.122.3.1038-1044.1975.

A hex mutant of Haemophilus influenzae.

Bagci H, STUY J Mol Gen Genet. 1979; 175(2):175-9.

PMID: 316097 DOI: 10.1007/BF00425533.

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