Ferritin-conjugated Antibodies Used for Labeling of Organelles Involved in the Cellular Synthesis and Transport of Procollagen

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1974 May 1

PMID 4525314

Citations 18

Authors

B R Olsen

D J Prockop

Affiliations

Soon will be listed here.

Abstract

An improved procedure was used to conjugate ferritin to antibodies specific for the NH(2)-terminal extensions on the precursor form of collagen known as procollagen. The ferritin-antibody conjugates were then used to determine the localization of procollagen in fibroblasts isolated from chick-embryo tendons. Procollagen was found in the cisternae of the endoplasmic reticulum, indicating that the protein passes into this compartment early in its biosynthesis. Specific labeling of large Golgi vacuoles was also observed, suggesting that this compartment is also involved in the secretory process. When cells were incubated with colchicine so that the secretion of procollagen was delayed, there was an increase of large, smooth-surfaced vacuoles in the cells and these vacuoles were labeled with the ferritin-antibody conjugate.

Citing Articles

Intracellular processing of procollagen induced by the action of colchicine.

Fernandez-Madrid F, Noonan S, Riddle J, Karvonen R, Sasaki D J Anat. 1980; 130(Pt 2):229-41.

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The "spindle-shaped" body in fibroblasts: intracellular collagen fibrils.

Fernandez-Madrid F, Noonan S, Riddle J J Anat. 1981; 132(Pt 2):157-66.

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Immunocytochemical localization of procollagen and fibronectin in human fibroblasts: effects of the monovalent ionophore, monensin.

Ledger P, Uchida N, Tanzer M J Cell Biol. 1980; 87(3 Pt 1):663-71.

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Immunological characterization of the major chick cartilage proteoglycan and its intracellular localization in cultured chondroblasts: a comparison with Type II procollagen.

Pacifici M, SOLTESZ R, Thal G, Shanley D, Boettiger D, Holtzer H J Cell Biol. 1983; 97(6):1724-36.

PMID: 6358233 PMC: 2112721. DOI: 10.1083/jcb.97.6.1724.

The secretion of tropoelastin by chick-embryo artery cells.

Saunders N, Grant M Biochem J. 1985; 230(1):217-25.

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