Transformation of Neuraminidase-treated Lymphocytes by Soybean Agglutinin

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1973 Sep 1

PMID 4517665

Citations 11

Authors

A Novogrodsky

E KATCHALSKI

Affiliations

Soon will be listed here.

Abstract

Transformation of mouse-spleen lymphocytes in the presence of soybean agglutinin is markedly enhanced after their treatment with neuraminidase (EC 3.2.1.18). Incubation of the cells with the enzyme also facilitates their agglutination by the lectin. The soybean agglutinin-induced agglutination and transformation is inhibited specifically by N-acetyl-D-galactosamine. New specific binding sites for soybean agglutinin were shown to appear after neuraminidase treatment. It is postulated that the transformation of neuraminidase-treated mouse-spleen lymphocytes induced by soybean agglutinin is caused by the binding of the lectin to galactosyl residues exposed by the action of neuraminidase on the cell membrane.

Citing Articles

Differential agglutination by soybean agglutinin of human leukemia and neuroblastoma cell lines: potential application to autologous bone marrow transplantation.

Reisner Y Proc Natl Acad Sci U S A. 1983; 80(21):6657-61.

PMID: 6356131 PMC: 391229. DOI: 10.1073/pnas.80.21.6657.

Thermodynamic and kinetic studies on saccharide binding to soya-bean agglutinin.

Swamy M, Krishna Sastry M, Khan M, Surolia A Biochem J. 1986; 234(3):515-22.

PMID: 3755041 PMC: 1146601. DOI: 10.1042/bj2340515.

Glycophorin A interferes in the agglutination of human erythrocytes by concanavalin A. Explanation of the requirement for enzymic predigestion.

Gokhale S, MEHTA N Biochem J. 1987; 241(2):505-11.

PMID: 3297033 PMC: 1147589. DOI: 10.1042/bj2410505.

The distinguishing characteristics of the plasma membrane are its exposed proteins.

Gates R, Phillips D, Morrison M Biochem J. 1975; 147(2):373-6.

PMID: 1180898 PMC: 1165454. DOI: 10.1042/bj1470373.

Mitogenic action of neuraminidase.

Semenzato G, SARASIN P, Amadori G, Gasparotto G Experientia. 1977; 33(11):1520-1.

PMID: 923734 DOI: 10.1007/BF01918846.

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