Rat Lymphocyte Mitogenesis by Aggregation Factor from Rat Ascites Hepatoma Cell Surface

Overview

Journal Br J Cancer

Specialty Oncology

Date 1978 Aug 1

PMID 359020

Citations 4

Authors

J Kuratsu

M Yoshinaga

H Hayashi

Affiliations

Soon will be listed here.

Abstract

Two-tumour-cell-aggregation factors derived from rat ascites hepatoma cells had different antigenicity; one, with a strong potency, was not absorbed by immunoadsorbent chromatography with anti-rat serum antibody and the other, with a weak potency, was. The unabsorbed factor possessed mitogenic activity on lymphocytes from thymus, spleen and lymph node of rats; its effect was compared with that of lectins (including phytohaemagglutinin, concanavalin A, pokeweed mitogen, lipopolysaccharide and soybean agglutinin) in the form of increased DNA and protein synthesis, blast transformation and mitosis. In the use of anti-thymocyte serum-resistant spleen cells and hydrocortisone-resistant thymocytes, the cells stimulated were assumed to be T-lymphocytes. DNA synthesis by this factor seemed to be characterized by a 2-step increase, suggesting the presence of 2 subpopulations of the cells activated, especially thymocytes. At high concentration this factor induced no depression of DNA synthesis. Favourable cell density for the response to this factor was 2-8 X 10(6) cells. Its effect was not influenced by treatment of the cells with neuraminidase.

Citing Articles

A monoclonal antibody disrupting cell-cell adhesion of rat ascites hepatoma cells.

Ohshima S, Ishimaru Y, Honda M, Ohkawara S, Ogawa M Cell Tissue Res. 1993; 273(2):363-70.

PMID: 8364973 DOI: 10.1007/BF00312839.

Immunological function of adhesive glycoprotein from rat ascites hepatoma cells: production of macrophage chemotactic lymphokine.

Hifumi M, Hayashi H Immunology. 1983; 49(2):245-53.

PMID: 6602088 PMC: 1454190.

Cell membrane polarity in rat ascites hepatoma cells. Distribution of a cell surface-associated adhesive factor on the cell surfaces.

Ishimaru Y, Hattori R, Chen J, Hayashi H Cell Tissue Res. 1985; 240(2):353-9.

PMID: 3995556 DOI: 10.1007/BF00222345.

An adhesive glycoprotein from rat ascites hepatoma cells potentiates natural cytotoxic activity by rat spleen cells.

Chen J, Kurano R, Hirashima M, Hayashi H Immunology. 1986; 58(1):95-100.

PMID: 3710521 PMC: 1452625.

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