Temperature-induced Variations in the Surface Topology of Cultured Lymphocytes Are Revealed by Scanning Electron Microscopy

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1973 Sep 1

PMID 4517661

Citations 24

Authors

P S Lin

D F Wallach

S Tsai

Affiliations

Soon will be listed here.

Abstract

Temperature-induced variations in the surface morphology of cultured lymphocytes were evaluated by scanning electron microscopy. At 25-37 degrees the cells' surfaces are largely obscured by numerous undulating microvilli of various lengths but uniform diameter. Temperature changes alter the number of microvilli, their lengths, diameters, distribution, branching, and fusing. Typically, chilling to 0-4 degrees markedly reduces the number of microvilli and increases the diameter of the survivors in a reversible process. In contrast, heating the cells to about 45 degrees rapidly and irreversibly transforms the ordinarily smooth membrane surface into one with a "cobblestone" morphology. At the same time most microvilli disappear and the few that remain clump into a cap. The data suggest that the low-temperature effects reflect a change in the physical state of membrane lipids, while the high-temperature alterations represent thermotropic protein transitions.

Citing Articles

Body temperature-related structural transitions of monotremal and human hemoglobin.

Digel I, Maggakis-Kelemen C, Zerlin K, Linder P, Kasischke N, Kayser P Biophys J. 2006; 91(8):3014-21.

PMID: 16844747 PMC: 1578488. DOI: 10.1529/biophysj.106.087809.

Internalization and catabolism of radiolabelled antibodies to the MHC class-II invariant chain by B-cell lymphomas.

Hansen H, Ong G, Diril H, Valdez A, Roche P, Griffiths G Biochem J. 1996; 320 ( Pt 1):293-300.

PMID: 8947500 PMC: 1217930. DOI: 10.1042/bj3200293.

Apical topography and modulation of ICAM-1 expression on activated endothelium.

Duperray A, Miles L, Felez J, Altieri D Am J Pathol. 1995; 147(5):1278-88.

PMID: 7485391 PMC: 1869507.

A comparison of the response to hyperthermia of murine haemopoietic stem cells (CFU-S) and L1210 leukaemia cells: enhanced killing of leukaemic cells in presence of normal marrow cells.

Symonds R, Wheldon T, Clarke B, Bailey G Br J Cancer. 1981; 44(5):682-91.

PMID: 7317271 PMC: 2010829. DOI: 10.1038/bjc.1981.254.

A review of cell surface markers and labelling techniques for scanning electron microscopy.

Molday R, Maher P Histochem J. 1980; 12(3):273-315.

PMID: 7002881 DOI: 10.1007/BF01006952.

References

Esfahani M, Limbrick A, Knutton S, Oka T, WAKIL S . The molecular organization of lipids in the membrane of Escherichia coli: phase transitions. Proc Natl Acad Sci U S A. 1971; 68(12):3180-4. PMC: 389617. DOI: 10.1073/pnas.68.12.3180. View

Lippert J, Peticolas W . Laser Raman investigation of the effect of cholesterol on conformational changes in dipalmitoyl lecithin multilayers. Proc Natl Acad Sci U S A. 1971; 68(7):1572-6. PMC: 389243. DOI: 10.1073/pnas.68.7.1572. View

Overath P, Schairer H, STOFFEL W . Correlation of in vivo and in vitro phase transitions of membrane lipids in Escherichia coli. Proc Natl Acad Sci U S A. 1970; 67(2):606-12. PMC: 283249. DOI: 10.1073/pnas.67.2.606. View

Melchior D, Morowitz H, STURTEVANT J, Tsong T . Characterization of the plasma membrane of Mycoplasma laidlawii. VII. Phase transitions of membrane lipids. Biochim Biophys Acta. 1970; 219(1):114-22. DOI: 10.1016/0005-2736(70)90066-0. View

Rotman B, PAPERMASTER B . Membrane properties of living mammalian cells as studied by enzymatic hydrolysis of fluorogenic esters. Proc Natl Acad Sci U S A. 1966; 55(1):134-41. PMC: 285766. DOI: 10.1073/pnas.55.1.134. View

STEIM J, Tourtellotte M, Reinert J, McElhaney R, Rader R . Calorimetric evidence for the liquid-crystalline state of lipids in a biomembrane. Proc Natl Acad Sci U S A. 1969; 63(1):104-9. PMC: 534007. DOI: 10.1073/pnas.63.1.104. View

Engelman D . X-ray diffraction studies of phase transitions in the membrane of Mycoplasma laidlawii. J Mol Biol. 1970; 47(1):115-7. DOI: 10.1016/0022-2836(70)90407-9. View

Loor F, Forni L, Pernis B . The dynamic state of the lymphocyte membrane. Factors affecting the distribution and turnover of surface immunoglobulins. Eur J Immunol. 1972; 2(3):203-12. DOI: 10.1002/eji.1830020304. View

Ladbrooke B, Chapman D . Thermal analysis of lipids, proteins and biological membranes. A review and summary of some recent studies. Chem Phys Lipids. 1969; 3(4):304-56. DOI: 10.1016/0009-3084(69)90040-1. View

10.

Blazyk J, STEIM J . Phase transitions in mammalian membranes. Biochim Biophys Acta. 1972; 266(3):737-41. DOI: 10.1016/0006-3002(72)90019-4. View

11.

Allan D, Crumpton M . Isolation and composition of human thymocyte plasma membrane. Biochim Biophys Acta. 1972; 274(1):22-7. DOI: 10.1016/0005-2736(72)90276-3. View

12.

FERBER E, Resch K, Wallach D, Imm W . Isolation and characterization of lymphocyte plasma membranes. Biochim Biophys Acta. 1972; 266(2):494-504. DOI: 10.1016/0005-2736(72)90105-8. View

13.

Leach S, Smith J . Thermal perturbation difference spectroscopy of proteins. Int J Protein Res. 1972; 4(1):11-9. DOI: 10.1111/j.1399-3011.1972.tb03393.x. View

14.

KOURILSKY F, Silvestre D, LEVY J, Dausset J, Nicolai M, Senik A . Immunoferritin study of the distribution of HL-A antigens on human blood cells. J Immunol. 1971; 106(2):454-66. View

15.

Weibel E . Stereological principles for morphometry in electron microscopic cytology. Int Rev Cytol. 1969; 26:235-302. DOI: 10.1016/s0074-7696(08)61637-x. View

16.

Engelman D . Lipid bilayer structure in the membrane of Mycoplasma laidlawii. J Mol Biol. 1971; 58(1):153-65. DOI: 10.1016/0022-2836(71)90238-5. View

17.

SHECHTER E, Gulik-Krzywicki T, Kaback H . Correlations between fluorescence, x-ray diffraction, and physiological properties in cytoplasmic membrane vesicles isolated from Escherichia coli. Biochim Biophys Acta. 1972; 274(2):466-77. DOI: 10.1016/0005-2736(72)90192-7. View

18.

Chapman D, Urbina J . Phase transitions and bilayer structure of Mycoplasma laidlawii B. FEBS Lett. 1971; 12(3):169-172. DOI: 10.1016/0014-5793(71)80060-1. View

19.

Allan D, Crumpton M . Preparation and characterization of the plasma membrane of pig lymphocytes. Biochem J. 1970; 120(1):133-43. PMC: 1179577. DOI: 10.1042/bj1200133. View

20.

Steck T . Cross-linking the major proteins of the isolated erythrocyte membrane. J Mol Biol. 1972; 66(2):295-305. DOI: 10.1016/0022-2836(72)90481-0. View