Distribution Pattern of Acid Phosphatase Activity in Human Peripheral Blood Leukocytes: a Cytochemical Scanning Electron Microscopy Study

Overview

Journal Histochemistry

Specialty Biochemistry

Date 1990 Jan 1

PMID 2401637

Citations 1

Authors

E Fernandez-Segura

J M Garcia

A Campos

Affiliations

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Abstract

The distribution patterns of acid phosphatase hydrolytic activity were studied in human peripheral blood cells with enzymocytochemical techniques together with light and scanning electron microscopy in the secondary and backscattered electron imaging modes. The acid phosphatase reaction product was seen in three different patterns of distribution: focal, granular and diffuse. These patterns were correlated with similar findings obtained with light microscopy. Acid phosphatase distribution patterns seen with SEM in the BEI mode were also correlated with the surface morphology of peripheral blood cells seen in the SEI mode. Cells exhibiting the focal pattern were smooth-surfaced with few microvilli; cells showing a granular pattern presented microvilli and microridges; ruffles were characteristic of cells with a diffuse pattern of activity. No reaction product was seen in cells bearing microvilli or ridges. Our findings demonstrate the correlation between acid phosphatase activity patterns and surface features in different subpopulations of peripheral blood cells.

Citing Articles

Dynamic reorganization of the alkaline phosphatase-containing compartment during chemotactic peptide stimulation of human neutrophils imaged by backscattered electrons.

Fernandez-Segura E, Garcia J, Campos A Histochem Cell Biol. 1995; 104(2):175-81.

PMID: 8536075 DOI: 10.1007/BF01451577.

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