A Comparison of the Response to Hyperthermia of Murine Haemopoietic Stem Cells (CFU-S) and L1210 Leukaemia Cells: Enhanced Killing of Leukaemic Cells in Presence of Normal Marrow Cells

Overview

Journal Br J Cancer

Specialty Oncology

Date 1981 Nov 1

PMID 7317271

Citations 4

Authors

R P Symonds

T E Wheldon

B Clarke

G Bailey

Affiliations

Soon will be listed here.

Abstract

When the clonogenic survival of mouse haemopoietic stem cells (CFU-S) and leukaemia L1210 cells growth as ascites tumours are compared after being heated in vitro and assayed in vivo by spleen-colony assay, there is no significant difference in the terminal slopes of the survival curves. The shoulders of the survival curves differ, but this may be explained by differences in cell kinetics. By contrast, L1210 leukaemic marrow cells are considerably more susceptible to the lethal effects of hyperthermia (43 degrees C) than either normal marrow stem cells or L1210 leukaemic cells grown as ascites tumours. Moreover, the killing of L1210 ascites cells by hyperthermia can be enhanced by heating L1210 ascites cells with an equal number of normal marrow cells, or as upernatant removed from heated marrow cells. Most cells in lukaemic marrow are normal, and it is postulated that the increased thermal sensitivity of L1210 cells in leukaemic marrow is caused by diffusible factors (e.g. lysosomal enzymes) released by heating normal marrow cells.

Citing Articles

Heat shock proteins and Bcl-2 expression and function in relation to the differential hyperthermic sensitivity between leukemic and normal hematopoietic cells.

Setroikromo R, Wierenga P, van Waarde M, Brunsting J, Vellenga E, Kampinga H Cell Stress Chaperones. 2008; 12(4):320-30.

PMID: 18229451 PMC: 2134794. DOI: 10.1379/csc-279.1.

Sensitivity of normal mouse marrow and RIF-1 tumour to hyperthermia combined with cyclophosphamide or BCNU: a lack of therapeutic gain.

Honess D, Bleehen N Br J Cancer. 1982; 46(2):236-48.

PMID: 6758828 PMC: 2011089. DOI: 10.1038/bjc.1982.189.

The sensitivity to hyperthermia of human granulocyte/macrophage progenitor cells (CFU-GM) derived from blood or marrow of normal subjects and patients with chronic granulocytic leukaemia.

Blackburn M, Wheldon T, FIELD S, Goldman J Br J Cancer. 1984; 50(6):745-51.

PMID: 6594154 PMC: 1977009. DOI: 10.1038/bjc.1984.251.

Thermal sensitivity of haemopoietic and stromal progenitor cells in different proliferative states.

Wang S, Hendry J, Testa N Br J Cancer. 1985; 52(1):51-4.

PMID: 4015952 PMC: 1977179. DOI: 10.1038/bjc.1985.147.

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