Bioassay of Oxytocin on the Superfused Mammary Gland of the Mouse, Using an Automatic Apparatus

Overview

Journal Br J Pharmacol

Publisher Wiley

Specialty Pharmacology

Date 1974 Mar 1

PMID 4368453

Citations 1

Authors

I C Robinson

J M Walker

Affiliations

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Abstract

1 A sensitive method of assaying oxytocin using the superfused mouse mammary gland is described. The method is shown to be reliable, accurate and precise. Assays are performed automatically and the preparation is stable for at least 18 hours.2 The preparation is relatively insensitive to bradykinin, histamine, 5-hydroxytryptamine, angiotensin, prostaglandin F(2alpha), adrenaline and noradrenaline. The contractions produced by acetylcholine can be abolished by atropine without the sensitivity to oxytocin being affected.3 Vasopressin has a variable activity on the preparation; its potency can be as high as one-fifth that of oxytocin.4 It is concluded that this method compares favourably with other published methods.

Citing Articles

Measuring Oxytocin and Vasopressin: Bioassays, Immunoassays and Random Numbers.

Leng G, Sabatier N J Neuroendocrinol. 2016; 28(10).

PMID: 27467712 PMC: 5096068. DOI: 10.1111/jne.12413.

References

Chard T, Forsling M, James M, Kitau M, LANDON J . The development of a radioimmunoassay for oxytocin: sensitivity of the assay in aqueous buffer solution, specificity and the dissociation of immunological and biological activity. J Endocrinol. 1970; 46(4):533-42. DOI: 10.1677/joe.0.0460533. View

Bisset G, Lewis G . A spectrum of pharmacological activity in some biologically active peptides. Br J Pharmacol Chemother. 1962; 19:168-82. PMC: 1482237. View

FOLLETT B, Bentley P . THE BIOASSAY OF OXYTOCIN: INCREASED SENSITIVITY OF THE RAT UTERUS IN RESPONSE TO SERIAL INJECTIONS OF STILBOESTROL. J Endocrinol. 1964; 29:277-82. DOI: 10.1677/joe.0.0290277. View

Van Dongen C, Hays R . A sensitive in vitro assay for oxytocin. Endocrinology. 1966; 78(1):1-6. DOI: 10.1210/endo-78-1-1. View

BANGHAM D, Mussett M . Third international standard for posterior pituitary; re-named third international standard for oxytocic, vasopressor and antidiuretic substances in 1956. Bull World Health Organ. 1958; 19(2):325-40. PMC: 2537666. View

FIELITZ C, Roca R, Mattei A, Melander S, Garofalo E, Gioia de Coch M . A sensitive and specific bioassay for oxytocin. Proc Soc Exp Biol Med. 1970; 133(4):1155-7. DOI: 10.3181/00379727-133-34643. View

Folley S, KNAGGS G . Levels of oxytocin in the jugular vein blood of goats during parturition. J Endocrinol. 1965; 33(2):301-15. DOI: 10.1677/joe.0.0330301. View

Chard T . The radioimmunoassay of oxytocin and vasopressin. J Endocrinol. 1973; 58(1):143-60. DOI: 10.1677/joe.0.0580143. View

Van Dongen C, Marshall J . Effect of various hormones on the milk ejection response of tissue isolated from the rat mammary gland. Nature. 1967; 213(5076):632-3. DOI: 10.1038/213632a0. View

10.

Bisset G, Clark B, Haldar J, Harris M, Lewis G, Rocha e Silva Jr R . The assay of milk-ejecting activity in the lactating rat. Br J Pharmacol Chemother. 1967; 31(3):537-49. PMC: 1557341. DOI: 10.1111/j.1476-5381.1967.tb00418.x. View

11.

TINDAL J, Yokoyama A . Assay of oxytocin by the milk-ejection response in the anesthetized lactating guinea pig. Endocrinology. 1962; 71:196-202. DOI: 10.1210/endo-71-2-196. View

12.

Bisset G, Errington M, Richards C . The distribution of vasopressin and oxytocin in the hypothalamoneurohypophysial system of the guinea-pig. Br J Pharmacol. 1973; 48(2):263-72. PMC: 1776216. DOI: 10.1111/j.1476-5381.1973.tb06912.x. View

13.

Mendez-Bauer C, Cabot H, Caldeyro-Barcia R . New Test for the Biological Assay of Oxytocin. Science. 1960; 132(3422):299-300. DOI: 10.1126/science.132.3422.299. View

14.

Bilek J, MIKULAS I, SLABA J, SKALA I . Bioassay of oxytocin by changes in light penetration through rat mammary gland tissue in vitro. J Endocrinol. 1971; 51(4):771-80. DOI: 10.1677/joe.0.0510771. View

15.

Fabian M, Forsling M, Jones J, Lee J . Comparison of two methods for the bioassay of oxytocin. Endocrinology. 1969; 85(3):600-3. DOI: 10.1210/endo-85-3-600. View

16.

Moore R, ZARROW M . CONTRACTION OF THE RABBIT MAMMARY STRIP IN VITRO IN RESPONSE TO OXYTOCIN. Acta Endocrinol (Copenh). 1965; 48:186-98. DOI: 10.1530/acta.0.0480186. View

17.

Roca R, Garofalo E, de Coch M, Arocena M, COCH J . Improvement in the bioassay of oxytocin by the mouse mammary gland in vitro. Proc Soc Exp Biol Med. 1972; 139(3):1010-2. DOI: 10.3181/00379727-139-36286. View

18.

Ryden G, Sjoholm I . Assay of oxytocin by rat mammary gland in vitro. Br J Pharmacol Chemother. 1962; 19:136-41. PMC: 1482239. DOI: 10.1111/j.1476-5381.1962.tb01434.x. View