Sequence of Events Mediating the Effect of Cholera Toxin on Rat Thymocytes

Overview

Journal J Clin Invest

Specialty General Medicine

Date 1974 Apr 1

PMID 4360858

Citations 10

Authors

J M Boyle

J D Gardner

Affiliations

Soon will be listed here.

Abstract

We have found that in rat thymocytes binding of [(125)I]choleragen is followed by cellular accumulation of cyclic 3',5'-AMP which, in turn, is followed by stimulation of amino acid transport. Binding of cholera toxin was complete by 30 min and remained constant for the subsequent 150 min. After stimulation by choleragen, cellular cyclic 3',5'-AMP became maximal by 30 min, after which it declined steadily so that by 90 min of incubation, cellular cyclic nucleotide levels were only 20% of those seen at 30 min. Stimulation of amino acid transport, although detectable by 15 min, did not become maximal until 120 min (by which time cellular cyclic 3',5'-AMP had decreased by more than 80%). We have also used this system to delineate the step at which various pharmacologic agents and hormones act to alter the sequence of events mediating the response of rat thymocytes to cholera toxin. The ability of cycloheximide to abolish choleragen-stimulated amino acid influx without reducing [(125)I]choleragen binding or cellular cyclic 3',5'-AMP suggests that cyclic nucleotide stimulation of amino acid transport includes a step involving protein synthesis.

Citing Articles

Comparison of the action of Escherichia coli enterotoxin on the thymocyte adenylate cyclase-cyclic adenosine monophosphate system to that of cholera toxin and prostaglandin E1.

Zenser T, METZGER J Infect Immun. 1974; 10(3):503-9.

PMID: 4372172 PMC: 422982. DOI: 10.1128/iai.10.3.503-509.1974.

Mode of action of cholera toxin: stabilization of catecholamine-sensitive adenylate cyclase in turkey erythrocytes.

Field M Proc Natl Acad Sci U S A. 1974; 71(8):3299-303.

PMID: 4370199 PMC: 388673. DOI: 10.1073/pnas.71.8.3299.

Action of cholecystokinin and cholinergic agents on calcium transport in isolated pancreatic acinar cells.

Gardner J, Conlon T, Kleveman H, Adams T, ONDETTI M J Clin Invest. 1975; 56(2):366-75.

PMID: 1150877 PMC: 436595. DOI: 10.1172/JCI108101.

Cytotoxic effect of Mycoplasma fermentans on mouse thymocytes.

Gabridge M, Schneider P Infect Immun. 1975; 11(3):460-5.

PMID: 1090532 PMC: 415086. DOI: 10.1128/iai.11.3.460-465.1975.

Mechanism of activation of adenylate cyclase by cholera toxin.

Sahyoun N, Cuatrecasas P Proc Natl Acad Sci U S A. 1975; 72(9):3438-42.

PMID: 1059129 PMC: 433009. DOI: 10.1073/pnas.72.9.3438.

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