The Biosynthesis of Phosphatidylinositol in Human Platelets

Overview

Journal J Clin Invest

Specialty General Medicine

Date 1970 Oct 1

PMID 4319007

Citations 6

Authors

C T Lucas

F L Call 2nd

W J Williams

Affiliations

Soon will be listed here.

Abstract

Homogenates of human platelets can mediate the synthesis of phosphatidylinositol from myoinositol and cytidine diphosphate diglyceride. The cytidine diphosphate diglyceride: myoinositol, phosphatidyl transferase activity is particulate-bound, and the highest specific activity is found in the membrane fraction. The production of phosphatidylinositol is decreased by sulfhydryl-binding agents, and the addition of thiols to the platelet homogenates increases the enzymatic activity. The reaction exhibits a pH optimum of 8.5-9.0. Divalent cations stimulate the reaction, and manganous chloride was the most effective of those investigated. The K(m) of the enzyme for myoinositol is 0.27 mM, and the K(m) for cytidine diphosphate diglyceride is 0.53 mM. The enzymatic activity of platelets isolated from patients with several diseases known to interfere with platelet clotpromoting function is similar to the enzymatic activity of platelets from normal donors.

Citing Articles

Phospholipid metabolism in stimulated human platelets. Changes in phosphatidylinositol, phosphatidic acid, and lysophospholipids.

Broekman M, Ward J, Marcus A J Clin Invest. 1980; 66(2):275-83.

PMID: 7400315 PMC: 371708. DOI: 10.1172/JCI109854.

Inositol lipids, phosphatidate and diacylglycerol share stearoylarachidonoylglycerol as a common backbone in thrombin-stimulated human platelets.

Mauco G, Dangelmaier C, Smith J Biochem J. 1984; 224(3):933-40.

PMID: 6525180 PMC: 1144530. DOI: 10.1042/bj2240933.

Cholinephosphotransferase activity in human platelets.

Goracci G, Gresele P, Arienti G, Porrovecchio P, Nenci G, Porcellati G Lipids. 1983; 18(3):179-85.

PMID: 6304446 DOI: 10.1007/BF02534545.

Phospholipid metabolism in human platelets.

Williams W, Call 2nd F Trans Am Clin Climatol Assoc. 1972; 83:71-80.

PMID: 4339148 PMC: 2441273.

Quantification of human platelet inositides and the influence of ionic environment on their incorporation of orthophosphate-32P.

Cohen P, Broekman M, Verkley A, Lisman J, Derksen A J Clin Invest. 1971; 50(4):762-72.

PMID: 4323126 PMC: 291990. DOI: 10.1172/JCI106547.

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