Cholinephosphotransferase Activity in Human Platelets

Overview

Journal Lipids

Specialty Biochemistry

Date 1983 Mar 1

PMID 6304446

Citations 3

Authors

G Goracci

P Gresele

G Arienti

P Porrovecchio

G G Nenci

G Porcellati

Affiliations

Soon will be listed here.

Abstract

Disrupted human platelets possess a cholinephosphotransferase activity (EC 2.7.8.2) whose properties have been studied in this work. The labeling of choline glycerophospholipid (CGP) from radioactive cytidine-5'-diphosphate choline (CDP-choline) in vitro shows a maximum at pH 8.0 (using Hepes [4-(2-hydroxyethyl)-piperazine-1-ethane-2-sulfonic acid] as a buffer) and is stimulated by Mn2+, Mg2+ and diacylglycerol. The enzymic activity is inhibited by Ca2+. The dependence of human platelet cholinephosphotransferase upon CDP-choline concentration does not follow the Michaelis-Menten equation. CMP strongly inhibits the reaction. The functional implications of this newly discovered platelet activity are briefly considered.

Citing Articles

Kinetic selectivity of cholinephosphotransferase in mouse liver: the Km for CDP-choline depends on diacylglycerol structure.

Mantel C, SCHULZ A, Miyazawa K, Broxmeyer H Biochem J. 1993; 289 ( Pt 3):815-20.

PMID: 8382052 PMC: 1132249. DOI: 10.1042/bj2890815.

Reversibility of cholinephosphotransferase in lung microsomes.

Tsao F Lipids. 1986; 21(8):498-502.

PMID: 3020334 DOI: 10.1007/BF02535636.

Lipid composition of platelets from patients with atherosclerosis: effect of purified eicosapentaenoic acid ethyl ester administration.

Kamido H, Matsuzawa Y, Tarui S Lipids. 1988; 23(10):917-23.

PMID: 2849012 DOI: 10.1007/BF02536337.

References

Smith J, Silver M, Webster G . Phospholipase A-1 of human blood platelets. Biochem J. 1973; 131(3):615-8. PMC: 1177510. DOI: 10.1042/bj1310615. View

Bereziat G, Chambaz J, Trugnan G, Pepin D, POLONOVSKI J . Turnover of phospholipid linoleic and arachidonic acids in human platelets from plasma lecithins. J Lipid Res. 1978; 19(4):495-500. View

Eisenthal R, Cornish-Bowden A . The direct linear plot. A new graphical procedure for estimating enzyme kinetic parameters. Biochem J. 1974; 139(3):715-20. PMC: 1166335. DOI: 10.1042/bj1390715. View

Chap H, DOUSTE-BLAZY L . [Phospholipase C-induced release reaction in platelets (author's transl)]. Eur J Biochem. 1974; 48(2):351-5. DOI: 10.1111/j.1432-1033.1974.tb03776.x. View

Kano H, Ono K . Utilization of endogenous phospholipids by the backreaction of CDP-choline (-ethanolamine): 1,2-diglyceride choline (ethanolamine)-phosphotransferase in rat liver microsomes. Biochim Biophys Acta. 1973; 306(2):203-17. View

Kannagi R, Koizumi K, Masuda T . Mobilization of arachidonic acid from phosphatidylethanolamine fraction to phosphatidylcholine fraction in platelets. Biochem Biophys Res Commun. 1980; 96(2):711-8. DOI: 10.1016/0006-291x(80)91413-8. View

Goracci G, Horrocks L, Porcellati G . Reversibility of ethanolamine and choline phosphotransferases (EC 2.7.8.1 and EC 2.7.8.2) in rat brain microsomes with labelled alkylacylglycerols. FEBS Lett. 1977; 80(1):41-4. DOI: 10.1016/0014-5793(77)80402-x. View

Marcus A, Safier L, ULLMAN H, Wong K, Broekman M, Weksler B . Effects of acetyl glyceryl ether phosphorylcholine on human platelet function in vitro. Blood. 1981; 58(5):1027-31. View

Ansell G, Metcalfe R . Studies on the CDP-ethanolamine-1,2-diglyceride ethanolaminephosphotransferase of rat brain. J Neurochem. 1971; 18(4):647-65. DOI: 10.1111/j.1471-4159.1971.tb11995.x. View

10.

. Production of diglyceride from phosphatidylinositol in activated human platelets. J Clin Invest. 1979; 63(4):580-7. PMC: 371991. DOI: 10.1172/JCI109339. View

11.

Shattil S, McDonough M, Burch J . Inhibition of platelet phospholipid methylation during platelet secretion. Blood. 1981; 57(3):537-44. View

12.

Binaglia L, Goracci G, Porcellati G, Roberti R, Woelk H . The synthesis of choline and ethanolamine phosphoglycerides in neuronal and glial cells of rabbit in vitro. J Neurochem. 1973; 21(5):1067-82. DOI: 10.1111/j.1471-4159.1973.tb07561.x. View

13.

Bell R, Kennerly D, Stanford N, MAJERUS P . Diglyceride lipase: a pathway for arachidonate release from human platelets. Proc Natl Acad Sci U S A. 1979; 76(7):3238-41. PMC: 383799. DOI: 10.1073/pnas.76.7.3238. View

14.

Goracci G, Francescangeli E, Horrocks L, Porcellati G . The reverse reaction of cholinephosphotransferase in rat brain microsomes. A new pathway for degradation of phosphatidylcholine. Biochim Biophys Acta. 1981; 664(2):373-9. DOI: 10.1016/0005-2760(81)90059-x. View

15.

Lucas C, Call 2nd F, Williams W . The biosynthesis of phosphatidylinositol in human platelets. J Clin Invest. 1970; 49(10):1949-55. PMC: 322685. DOI: 10.1172/JCI106414. View

16.

. Indomethacin-induced accumulation of diglyceride in activated human platelets. The role of diglyceride lipase. J Biol Chem. 1980; 255(6):2259-62. View

17.

Call 2nd F, Rubert M . Synthesis of ethanolamine phosphoglycerides by human platelets. J Lipid Res. 1975; 16(5):352-9. View

18.

Elsbach P, Pettis P, Marcus A . Lysolecithin metabolism by human platelets. Blood. 1971; 37(6):675-83. View

19.

Billah M, Lapetina E, Cuatrecasas P . Phospholipase A2 and phospholipase C activities of platelets. Differential substrate specificity, Ca2+ requirement, pH dependence, and cellular localization. J Biol Chem. 1980; 255(21):10227-31. View

20.

Derksen A, Cohen P . Patterns of fatty acid release from endogenous substrates by human platelet homogenates and membranes. J Biol Chem. 1975; 250(24):9342-7. View