Vitamin D and Adenosine Triphosphatase Dependent on Divalent Cations in Rat Intestinal Mucosa

Overview

Journal J Clin Invest

Specialty General Medicine

Date 1973 Nov 1

PMID 4270644

Citations 9

Authors

S KOWARSKI

D Schachter

Affiliations

Soon will be listed here.

Abstract

Intestinal brush borders prepared from vitamin D-deficient rats demonstrate increased susceptibility in vitro to fragmentation by shear forces or to loss of microvillus enzymes on treatment with EDTA. These effects are relatively nonspecific and are also observed in normal rats starved for 48 h. They may underlie prior observations that purport to demonstrate a vitamin D-dependent increase in brush border Ca-dependent ATPase. In addition, however, vitamin D increases ATPase activity dependent on certain divalent cations, including Ca and Zn, in whole-particulate suspensions pelleted by high-speed centrifugation of mucosal homogenates. This action is independent of changes in other microvillus enzymes, i.e. disaccharidases, and tissue distribution and cation specificity studies support the hypothesis that the mucosal whole-particulate ATPase is related to transport of Ca, Zn, and possibly other divalent cations.

Citing Articles

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Effects of cortisol and fluoride on ion-transporting ATPase activities in cultured osteoblastlike cells.

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Ion-transporting ATPases and matrix mineralization in cultured osteoblastlike cells.

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Calcium translocation by Golgi and lateral-basal membrane vesicles from rat intestine: decrease in vitamin D-deficient rats.

Freedman R, Weiser M, Isselbacher K Proc Natl Acad Sci U S A. 1977; 74(8):3612-6.

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