Simple, Inexpensive Procedure for the Disruption of Bacteria

Overview

Journal Appl Microbiol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1974 Jul 1

PMID 4210727

Citations 13

Authors

J M Ranhand

Affiliations

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Abstract

Small volumes (1 to 2 ml) of bacterial cultures, with turbidities ranging from 3 to 10, were disrupted 50 to 90% (measured as a decrease in turbidity) within 2 min, by shaking them on a Vortex-type mixer in the presence of glass beads. This method of disrupton was effective for cells in the following genera: Streptococcus, Lactobacillus, Staphylococcus, Bacillus, and Escherichia. After low-speed centrifugation, the resulting extract can be used as potential sources for enzymes, transforming deoxyribonucleic acids, cell walls, membranes, etc.

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References

Ranhand J, Leonard C, Cole R . Autolytic activity associated with competent group H streptococci. J Bacteriol. 1971; 106(1):257-68. PMC: 248670. DOI: 10.1128/jb.106.1.257-268.1971. View

Ranhand J, Cole R . Demonstration of transforming deoxyribonucleic acid in an autolysate of a group H Streptococcus. J Bacteriol. 1971; 106(2):712-3. PMC: 285156. DOI: 10.1128/jb.106.2.712-713.1971. View

Parsons L, Ranhand J, Leonard C, Colon A, Cole R . Inhibition of transformation in group H streptococci by lysogeny. J Bacteriol. 1973; 113(3):1217-22. PMC: 251685. DOI: 10.1128/jb.113.3.1217-1222.1973. View

Ranhand J . Autolytic activity and its association with the development of competence in group H streptococci. J Bacteriol. 1973; 115(2):607-14. PMC: 246289. DOI: 10.1128/jb.115.2.607-614.1973. View

Hahn J, Cole R . Time and concentration relationships in the long-chain reaction of group A streptococci in homologous antiserum and an improved method for evaluation of test results. J Bacteriol. 1962; 83:85-96. PMC: 314792. DOI: 10.1128/jb.83.1.85-96.1962. View