Australia Antigen (hepatitis B Antigen): a Conformational Antigen Dependent on Disulfide Bonds

Overview

Journal Science

Specialty Science

Date 1972 Dec 22

PMID 4118259

Citations 22

Authors

G N VYAS

K R Rao

A B Ibrahim

Affiliations

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Abstract

Reduction and alkylation of purified hepatitis-associated Australia antigen (hepatitis B antigen) resulted in a total loss of serologic activity. The reduced and alkylated protein formed a single band with a sedimentation coefficient of 31S on analytical ultracentrifugation, and no subunits were detected by Sephadex gel filtration. Although this preparation induced a delayed hypersensitivity response when injected into guinea pigs, it failed to stimulate humoral antibody formation. The data suggest that hepatitis B antigen is a conformational antigen critically dependent upon the disulfide bonds of the protein moiety.

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