Chemically Synthesized Peptides Predicted from the Nucleotide Sequence of the Hepatitis B Virus Genome Elicit Antibodies Reactive with the Native Envelope Protein of Dane Particles

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1981 Jun 1

PMID 6167985

Citations 97

Authors

R A Lerner

N Green

H Alexander

F T Liu

J G Sutcliffe

T M Shinnick

Affiliations

Soon will be listed here.

Abstract

Thirteen peptides corresponding to amino acid sequences predicted from the nucleotide sequence of the hepatitis B surface antigen were synthesized chemically. The free or carrier-linked synthetic peptides were injected into rabbits, and 7 of the 13 elicited an antipeptide response. Antisera against four of the six soluble peptides longer than 10 amino acids were reactive with native antigen and specifically precipitated the 23,000- and 28,000-dalton forms from Dane particles. As the hepatitis molecule had not been chosen for study because of any structural feature suggesting unique opportunities for success, these results suggest that the strategy is general and should work for any protein as long as enough domains are studied. Peptides such as these could prove to be ideal vaccines.

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References

Galibert F, Mandart E, Fitoussi F, Tiollais P, Charnay P . Nucleotide sequence of the hepatitis B virus genome (subtype ayw) cloned in E. coli. Nature. 1979; 281(5733):646-50. DOI: 10.1038/281646a0. View

Koistinen V . Hepatitis B surface antigen polypeptides: artifactual bands in sodium dodecyl sulfate-polyacrylamide gel electrophoresis caused by aggregation. J Virol. 1980; 35(1):20-3. PMC: 288778. DOI: 10.1128/JVI.35.1.20-23.1980. View

Sutcliffe J, Shinnick T, Green N, Liu F, Niman H, Lerner R . Chemical synthesis of a polypeptide predicted from nucleotide sequence allows detection of a new retroviral gene product. Nature. 1980; 287(5785):801-5. DOI: 10.1038/287801a0. View

Walter G, Scheidtmann K, Carbone A, Laudano A, Doolittle R . Antibodies specific for the carboxy- and amino-terminal regions of simian virus 40 large tumor antigen. Proc Natl Acad Sci U S A. 1980; 77(9):5197-200. PMC: 350024. DOI: 10.1073/pnas.77.9.5197. View

Arnon R . Chemically defined antiviral vaccines. Annu Rev Microbiol. 1980; 34:593-618. DOI: 10.1146/annurev.mi.34.100180.003113. View

Wilson I, Skehel J, Wiley D . Structure of the haemagglutinin membrane glycoprotein of influenza virus at 3 A resolution. Nature. 1981; 289(5796):366-73. DOI: 10.1038/289366a0. View

Audibert F, Jolivet M, CHEDID L, ALOUF J, Boquet P, Rivaille P . Active antitoxic immunization by a diphtheria toxin synthetic oligopeptide. Nature. 1981; 289(5798):593-4. DOI: 10.1038/289593a0. View

Ellman G . Tissue sulfhydryl groups. Arch Biochem Biophys. 1959; 82(1):70-7. DOI: 10.1016/0003-9861(59)90090-6. View

DANE D, Cameron C, Briggs M . Virus-like particles in serum of patients with Australia-antigen-associated hepatitis. Lancet. 1970; 1(7649):695-8. DOI: 10.1016/s0140-6736(70)90926-8. View

10.

Millman I, Loeb L, Bayer M, Blumberg B . Australia antigen (a hepatitis-associated antigen): purification and physical properties. J Exp Med. 1970; 131(6):1190-9. PMC: 2138848. DOI: 10.1084/jem.131.6.1190. View

11.

Marglin A, MERRIFIELD R . Chemical synthesis of peptides and proteins. Annu Rev Biochem. 1970; 39:841-66. DOI: 10.1146/annurev.bi.39.070170.004205. View

12.

VYAS G, Rao K, Ibrahim A . Australia antigen (hepatitis B antigen): a conformational antigen dependent on disulfide bonds. Science. 1972; 178(4067):1300-1. DOI: 10.1126/science.178.4067.1300. View

13.

Sachs D, Schechter A, Eastlake A, ANFINSEN C . An immunologic approach to the conformational equilibria of polypeptides. Proc Natl Acad Sci U S A. 1972; 69(12):3790-4. PMC: 389874. DOI: 10.1073/pnas.69.12.3790. View

14.

Shih J, Gerin J . Proteins of hepatitis B surface antigen. J Virol. 1977; 21(1):347-57. PMC: 353821. DOI: 10.1128/JVI.21.1.347-357.1977. View

15.

Peterson D, Roberts I, VYAS G . Partial amino acid sequence of two major component polypeptides of hepatitis B surface antigen. Proc Natl Acad Sci U S A. 1977; 74(4):1530-4. PMC: 430823. DOI: 10.1073/pnas.74.4.1530. View

16.

Liu F, Zinnecker M, Hamaoka T, Katz D . New procedures for preparation and isolation of conjugates of proteins and a synthetic copolymer of D-amino acids and immunochemical characterization of such conjugates. Biochemistry. 1979; 18(4):690-3. DOI: 10.1021/bi00571a022. View

17.

Valenzuela P, Gray P, Quiroga M, Zaldivar J, Goodman H, Rutter W . Nucleotide sequence of the gene coding for the major protein of hepatitis B virus surface antigen. Nature. 1979; 280(5725):815-9. DOI: 10.1038/280815a0. View

18.

Mishiro S, Imai M, Takahashi K, Machida A, Gotanda T, Miyakawa Y . A 49,000-dalton polypeptide bearing all antigenic determinants and full immunogenicity of 22-nm hepatitis B surface antigen particles. J Immunol. 1980; 124(4):1589-93. View

19.

Pasek M, Goto T, Gilbert W, Zink B, Schaller H, MacKay P . Hepatitis B virus genes and their expression in E. coli. Nature. 1979; 282(5739):575-9. DOI: 10.1038/282575a0. View