Amino Acid Sequence of the Encephalitogenic Basic Protein from Human Myelin

Overview

Journal Biochem J

Specialty Biochemistry

Date 1971 Jun 1

PMID 4108501

Citations 37

Authors

P R Carnegie

Affiliations

Soon will be listed here.

Abstract

Myelin from the central nervous system contains an unusual basic protein, which can induce experimental autoimmune encephalomyelitis. The basic protein from human brain was digested with trypsin and other enzymes and the sequence of the 170 amino acids was determined. The localization of the encephalitogenic determinants was described. Possible roles for the protein in the structure and function of myelin are discussed.

Citing Articles

Multiple sclerosis and myelin basic protein: insights into protein disorder and disease.

Martinsen V, Kursula P Amino Acids. 2021; 54(1):99-109.

PMID: 34889995 PMC: 8810476. DOI: 10.1007/s00726-021-03111-7.

An Overview of the Intrinsic Role of Citrullination in Autoimmune Disorders.

Alghamdi M, Alasmari D, Assiri A, Mattar E, Aljaddawi A, Alattas S J Immunol Res. 2019; 2019:7592851.

PMID: 31886309 PMC: 6899306. DOI: 10.1155/2019/7592851.

Mesenchymal Stem Cell-Based Immunomodulation: Properties and Clinical Application.

Wang M, Yuan Q, Xie L Stem Cells Int. 2018; 2018:3057624.

PMID: 30013600 PMC: 6022321. DOI: 10.1155/2018/3057624.

Human mesenchymal stem cells (MSCs) for treatment towards immune- and inflammation-mediated diseases: review of current clinical trials.

Wang L, Ting C, Yen M, Liu K, Sytwu H, Wu K J Biomed Sci. 2016; 23(1):76.

PMID: 27809910 PMC: 5095977. DOI: 10.1186/s12929-016-0289-5.

The role of proteolytic enzymes in demyelination in experimental allergic encephalomyelitis.

Smith M Neurochem Res. 2013; 2(3):233-46.

PMID: 24272051 DOI: 10.1007/BF00969354.

References

Carnegie P . Digestion of an arg-pro bond by trypsin in the ecephalitogenic basic protein of human myelin. Nature. 1969; 223(5209):958-9. DOI: 10.1038/223958a0. View

Swaney J, KLOTZ I . Amino acid sequence adjoining the lone tryptophan of human serum albumin. A binding site of the protein. Biochemistry. 1970; 9(13):2570-4. DOI: 10.1021/bi00815a003. View

Agrawal H, Banik N, Bone A, Davison A, Mitchell R, Spohn M . The identity of a myelin-like fraction isolated from developing brain. Biochem J. 1970; 120(3):635-42. PMC: 1179645. DOI: 10.1042/bj1200635. View

Carnegie P, McPherson T, Robson G . Experimental autoimmune encephalomyelitis. Digestion of basic protein of human myelin with cyanogen bromide and trypsin. Immunology. 1970; 19(1):55-63. PMC: 1455612. View

KIBLER R, Shapira R, McKneally S, Jenkins J, Selden P, Chou F . Encephalitogenic protein: structure. Science. 1969; 164(3879):577-80. DOI: 10.1126/science.164.3879.577. View

WALEY S, Watson J . The action of trypsin on polylysine. Biochem J. 1953; 55(2):328-37. PMC: 1269242. DOI: 10.1042/bj0550328. View

Hashim G, Eylar E . The structure of the terminal regions of the encephalitogenic basic protein from bovine myelin. Arch Biochem Biophys. 1969; 135(1):324-33. DOI: 10.1016/0003-9861(69)90546-3. View

Blow D, Birktoft J, Hartley B . Role of a buried acid group in the mechanism of action of chymotrypsin. Nature. 1969; 221(5178):337-40. DOI: 10.1038/221337a0. View

Baldwin G, Carnegie P . Isolation and partial characterization of methylated arginines from the encephalitogenic basic protein of myelin. Biochem J. 1971; 123(1):69-74. PMC: 1176900. DOI: 10.1042/bj1230069. View

10.

Milstein C . A simple procedure for the fractionation of the tryptic peptides of the C-terminal half of immunoglobulin lambda-chains. Biochem J. 1968; 110(4):652-4. PMC: 1187435. DOI: 10.1042/bj1100652. View

11.

Hartley B . Strategy and tactics in protein chemistry. Biochem J. 1970; 119(5):805-22. PMC: 1179482. DOI: 10.1042/bj1190805f. View

12.

Chao L, EINSTEIN E . Physical properties of the bovine encephalitogenic protein; molecular weight and conformation. J Neurochem. 1970; 17(8):1121-32. DOI: 10.1111/j.1471-4159.1970.tb03360.x. View

13.

Palmer F, Dawson R . The isolation and properties of experimental allergic encephalitogenic protein. Biochem J. 1969; 111(5):629-36. PMC: 1187591. DOI: 10.1042/bj1110629. View

14.

Ambler R, Meadway R . The use of thermolysin in amino acid sequence determination. Biochem J. 1968; 108(5):893-5. PMC: 1198899. DOI: 10.1042/bj1080893. View

15.

Westall F, Robinson A, Caccam J, Jackson J, Ylar E . Essential chemical requirements for induction of allergic encephalomyelitis. Nature. 1971; 229(5279):22-4. DOI: 10.1038/229022a0. View

16.

Lennon V, Wilks A, Carnegie P . Immunologic properties of the main encephalitogenic peptide from the basic protein of human myelin. J Immunol. 1970; 105(5):1223-30. View

17.

Eylar E, Hashim G . Allergic encephalomyelitis: the structure of encephalitogenic determinant. Proc Natl Acad Sci U S A. 1968; 61(2):644-50. PMC: 225208. DOI: 10.1073/pnas.61.2.644. View

18.

Field E, CASPARY E . Lymphocyte sensitisation: an in-vitro test for cancer?. Lancet. 1970; 2(7687):1337-41. DOI: 10.1016/s0140-6736(70)92361-5. View

19.

CASPARY E, Field E . AN ENCEPHALITOGENIC PROTEIN OF HUMAN ORIGIN: SOME CHEMICAL AND BIOLOGICAL PROPERTIES. Ann N Y Acad Sci. 1965; 122:182-98. DOI: 10.1111/j.1749-6632.1965.tb20202.x. View

20.

Field E, Raine C, Hughes D . Failure to induce myelin sheath formation around artificial fibres with a note on the toxicity of polyester fibres for nervous tissue in vitrol. J Neurol Sci. 1969; 8(1):129-41. DOI: 10.1016/0022-510x(69)90046-x. View