Live-Cell Single-Molecule Imaging of Influenza A Virus-Receptor Interaction

Overview

Journal Methods Mol Biol

Date 2025 Jan 31

PMID 39890722

Authors

Lukas Broich

Yang Fu

Christian Sieben

Affiliations

Soon will be listed here.

Abstract

Influenza A viruses are a major health care burden, and their biology has been intensely studied for decades. However, many details of virus infection are still elusive, requiring the development of refined and advanced technologies. Super-resolution microscopy allows the study of virus replication at the scale of an infecting virus, offering an exciting perspective on previously unseen mechanistic details of infection. Here we describe the materials and procedures required to perform single-molecule imaging of virus-receptor interaction in live cells. We further provide hints and tips on how to analyze and visualize the obtained datasets.

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