High-density Mapping of Single-molecule Trajectories with Photoactivated Localization Microscopy

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2008 Jan 15

PMID 18193054

Citations 523

Authors

Suliana Manley

Jennifer M Gillette

George H Patterson

Hari Shroff

Harald F Hess

Eric Betzig

Jennifer Lippincott-Schwartz

Affiliations

Soon will be listed here.

Abstract

We combined photoactivated localization microscopy (PALM) with live-cell single-particle tracking to create a new method termed sptPALM. We created spatially resolved maps of single-molecule motions by imaging the membrane proteins Gag and VSVG, and obtained several orders of magnitude more trajectories per cell than traditional single-particle tracking enables. By probing distinct subsets of molecules, sptPALM can provide insight into the origins of spatial and temporal heterogeneities in membranes.

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