Red Currant ( L.) Fruit Waste Extract and Juice As Potential Spasmolytic Agents

Overview

Journal Plants (Basel)

Date 2025 Jan 25

PMID 39861587

Authors

Maja Cvetkovic

Bojana Miladinovic

Suzana Brankovic

Milica Randjelovic

Slavoljub Zivanovic

Nemanja Kitic

Milica Milutinovic

Milos Jovanovic

Dusica Stojanovic

Haris Niksic

Katarina Savikin

Dusanka Kitic

Affiliations

Soon will be listed here.

Abstract

Red currant ( L.) berries are rich in bioactive compounds and exhibit nutritive and protective features. This research examined the content of secondary metabolites of the red currant variety Redpoll lyophilized juice and waste extract and their antioxidative and spasmolytic effects. The flavonol and anthocyanin contents of the samples were determined using high-performance liquid chromatography. The antispasmodic effects were assessed in in vitro conditions, and the potential impact of the samples and possible action mechanisms were revealed. The results showed the prevalence of cyanidin-3--glucoside as the dominant anthocyanin with higher content in the juice sample. Quercetin content, as the prevalent flavonol, was higher in the waste sample. The berry juice showed a greater ability for scavenging free radicals, whereas the waste better inhibited lipid peroxidation. The juice was a superior antispasmodic agent for spontaneous, KCl-, CaCl-, BaCl-, histamine-, and acetylcholine-induced ileal contractions. This first evaluation of the red currant variety Redpoll lyophilized juice and waste extract indicated the beneficial effects of juice as an antioxidant and spasmolytic agent. Therefore, this red currant juice sample could be designated for the prevention or treatment of gastrointestinal disorders.

References

Zoli M, Pucci S, Vilella A, Gotti C . Neuronal and Extraneuronal Nicotinic Acetylcholine Receptors. Curr Neuropharmacol. 2017; 16(4):338-349. PMC: 6018187. DOI: 10.2174/1570159X15666170912110450. View

Frountzas M, Karanikki E, Toutouza O, Sotirakis D, Schizas D, Theofilis P . Exploring the Impact of Cyanidin-3-Glucoside on Inflammatory Bowel Diseases: Investigating New Mechanisms for Emerging Interventions. Int J Mol Sci. 2023; 24(11). PMC: 10254033. DOI: 10.3390/ijms24119399. View

Rees A, Dodd G, Spencer J . The Effects of Flavonoids on Cardiovascular Health: A Review of Human Intervention Trials and Implications for Cerebrovascular Function. Nutrients. 2018; 10(12). PMC: 6315948. DOI: 10.3390/nu10121852. View

Knekt P, Jarvinen R, Seppanen R, Hellovaara M, Teppo L, Pukkala E . Dietary flavonoids and the risk of lung cancer and other malignant neoplasms. Am J Epidemiol. 1997; 146(3):223-30. DOI: 10.1093/oxfordjournals.aje.a009257. View

Liu S, Hu H, Ren J, Gao C, Gao N, Lin Z . Pre- and postsynaptic inhibition by nociceptin in guinea pig small intestinal myenteric plexus in vitro. Am J Physiol Gastrointest Liver Physiol. 2001; 281(1):G237-46. DOI: 10.1152/ajpgi.2001.281.1.G237. View

Larson A, Symons J, Jalili T . Quercetin: A Treatment for Hypertension?-A Review of Efficacy and Mechanisms. Pharmaceuticals (Basel). 2016; 3(1):237-250. PMC: 3991028. DOI: 10.3390/ph3010237. View

Imran M, Rauf A, Shah Z, Saeed F, Imran A, Arshad M . Chemo-preventive and therapeutic effect of the dietary flavonoid kaempferol: A comprehensive review. Phytother Res. 2018; 33(2):263-275. DOI: 10.1002/ptr.6227. View

Milivojevic J, Slatnar A, Mikulic-Petkovsek M, Stampar F, Nikolic M, Veberic R . The influence of early yield on the accumulation of major taste and health-related compounds in black and red currant cultivars (Ribes spp.). J Agric Food Chem. 2012; 60(10):2682-91. DOI: 10.1021/jf204627m. View

Cujic N, Savikin K, Jankovic T, Pljevljakusic D, Zdunic G, Ibric S . Optimization of polyphenols extraction from dried chokeberry using maceration as traditional technique. Food Chem. 2015; 194:135-42. DOI: 10.1016/j.foodchem.2015.08.008. View

10.

Mikulic Petkovsek M, Slatnar A, Stampar F, Veberic R . The influence of organic/integrated production on the content of phenolic compounds in apple leaves and fruits in four different varieties over a 2-year period. J Sci Food Agric. 2010; 90(14):2366-78. DOI: 10.1002/jsfa.4093. View

11.

Drossman D . Functional Gastrointestinal Disorders: History, Pathophysiology, Clinical Features and Rome IV. Gastroenterology. 2016; . DOI: 10.1053/j.gastro.2016.02.032. View

12.

MASUZAWA K, Matsuda T, Asano M . Evidence that pinacidil may promote the opening of ATP-sensitive K+ channels yet inhibit the opening of Ca2(+)-activated K+ channels in K(+)-contracted canine mesenteric artery. Br J Pharmacol. 1990; 100(1):143-9. PMC: 1917474. DOI: 10.1111/j.1476-5381.1990.tb12066.x. View

13.

Ren J, Lu Y, Qian Y, Chen B, Wu T, Ji G . Recent progress regarding kaempferol for the treatment of various diseases. Exp Ther Med. 2019; 18(4):2759-2776. PMC: 6755486. DOI: 10.3892/etm.2019.7886. View

14.

Hemming J, Guarraci F, Firth T, Jennings L, Nelson M, Mawe G . Actions of histamine on muscle and ganglia of the guinea pig gallbladder. Am J Physiol Gastrointest Liver Physiol. 2000; 279(3):G622-30. DOI: 10.1152/ajpgi.2000.279.3.G622. View

15.

Miladinovic B, Brankovic S, Kostic M, Milutinovic M, Kitic N, Savikin K . Antispasmodic Effect of Blackcurrant (Ribes nigrum L.) Juice and Its Potential Use as Functional Food in Gastrointestinal Disorders. Med Princ Pract. 2018; 27(2):179-185. PMC: 5968245. DOI: 10.1159/000487202. View

16.

Macander P . Flavonoids affect acetylcholine, prostaglandin E2 and antigen-mediated smooth muscle contraction. Prog Clin Biol Res. 1986; 213:489-92. View

17.

Yoro Sy G, Sarr A, Dieye A, Faye B . Myorelaxant and antispasmodic effects of the aqueous extract of Mitragyna inermis barks on Wistar rat ileum. Fitoterapia. 2004; 75(5):447-50. DOI: 10.1016/j.fitote.2004.03.006. View

18.

Zamora-Ros R, Forouhi N, Sharp S, Gonzalez C, Buijsse B, Guevara M . Dietary intakes of individual flavanols and flavonols are inversely associated with incident type 2 diabetes in European populations. J Nutr. 2013; 144(3):335-43. PMC: 3927546. DOI: 10.3945/jn.113.184945. View

19.

Santos-Fagundes D, Grasa L, Gonzalo S, Valero M, Castro M, Arruebo M . Different mechanisms of actions of genistein, quercetin on spontaneous contractions of rabbit duodenum. Rev Esp Enferm Dig. 2015; 107(7):413-6. DOI: 10.17235/reed.2015.3695/2015. View

20.

Du G, Sun L, Zhao R, Du L, Song J, Zhang L . Polyphenols: Potential source of drugs for the treatment of ischaemic heart disease. Pharmacol Ther. 2016; 162:23-34. DOI: 10.1016/j.pharmthera.2016.04.008. View