Expanding the Immune-related Targetome of MiR-155-5p by Integrating Time-resolved RNA Patterns into MiRNA Target Prediction

Overview

Journal RNA Biol

Specialty Molecular Biology

Date 2025 Jan 6

PMID 39760255

Authors

Martin Hart

Caroline Diener

Stefanie Rheinheimer

Tim Kehl

Andreas Keller

Hans-Peter Lenhof

Eckart Meese

Affiliations

Soon will be listed here.

Abstract

The lack of a sufficient number of validated miRNA targets severely hampers the understanding of their biological function. Even for the well-studied miR-155-5p, there are only 239 experimentally validated targets out of 42,554 predicted targets. For a more complete assessment of the immune-related miR-155 targetome, we used an inverse correlation of time-resolved mRNA profiles and miR-155-5p expression of early CD4+ T cell activation to predict immune-related target genes. Using a high-throughput miRNA interaction reporter (HiTmIR) assay we examined 90 target genes and confirmed 80 genes as direct targets of miR-155-5p. Our study increases the current number of verified miR-155-5p targets approximately threefold and exemplifies a method for verifying miRNA targetomes as a prerequisite for the analysis of miRNA-regulated cellular networks.

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