Functional Modulation of Gut Microbiota and Blood Parameters in Diabetic Rats Following Dietary Intervention with Free or Immobilized SK Cells on Pistachio Nuts

Overview

Journal Nutrients

Date 2024 Dec 17

PMID 39683613

Authors

Ioanna Prapa

Amalia E Yanni

Vasiliki Kompoura

Gregoria Mitropoulou

Panayiotis Panas

Nikolaos Kostomitsopoulos

Yiannis Kourkoutas

Affiliations

Soon will be listed here.

Abstract

Background/objectives: The gut microbiota is linked to the pathogenesis of type 1 diabetes mellitus (T1DM), while supplementation with probiotics may result in positive alterations in the composition of the gut microbiome. This research aimed to map the changes in the gut microbiome and blood markers of streptozotocin-induced diabetic rats after a dietary intervention with free or immobilized cells of the presumptive probiotic SK on pistachio nuts.

Methods: Twenty-four male Wistar rats were studied and divided into four groups (healthy or diabetic) which received the free or the immobilized . SK cells on pistachio nuts for 4 weeks. Blood, fecal, and intestinal tissue samples were examined.

Results: The diabetic rats exhibited an elevated concentration of HDL-c, while the inflammatory IL-1β levels were significantly lower in the diabetic animals that received the immobilized cells compared to the group that received the free cells. The dietary intervention with immobilized cells led to decreased counts of fecal staphylococci and enterococci in the diabetic animals, while the diet with both free and immobilized . SK cells rendered levels of these populations in normal values in the feces and intestinal tissue of the diabetic animals. Noticeably, the and genera were elevated after the supplementation with immobilized SK cells on pistachio nuts.

Conclusions: Dietary supplementation with SK cells (in free or in immobilized form) beneficially affected the gut microbiota/microbiome of streptozotocin-induced diabetic rats, leading to the alleviation of dysbiosis and inflammation and control over their lipid levels.

Citing Articles

Assessment of Immobilized OLXAL-1 Cells on Oat Flakes for Functional Regulation of the Intestinal Microbiome in a Type-1 Diabetic Animal Model.

Nelios G, Prapa I, Mitropoulou G, Kompoura V, Balafas E, Kostomitsopoulos N Foods. 2025; 13(24.

PMID: 39767077 PMC: 11675650. DOI: 10.3390/foods13244134.

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